Advancing Thermophilic Anaerobic Digestion of Corn Whole Stillage: Lignocellulose Decomposition and Microbial Community Characterization

Fermentation Pub Date : 2024-06-08 DOI:10.3390/fermentation10060306

A. Bokhary, Fuad Ale Enriquez, Richard Garrison, B. Ahring

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Abstract

Converting corn grains into bioethanol is an expanding practice for sustainable fuel production, but this is accompanied by the production of large quantities of by-products such as whole stillage. In the present study, the influence of advanced wet oxidation and steam explosion (AWOEx) pretreatment on biogas production and lignocellulose decomposition of corn whole stillage (CWS) was evaluated using semi-continuous thermophilic reactors. The digestion of the CWS was shown to be feasible with an organic loading rate (OLR) of 1.12 ± 0.03 kg VS/m3 day and a hydraulic retention time (HRT) of 30 days, achieving a methane yield of 0.75 ± 0.05 L CH4/g VSfed for untreated stillage and 0.86 ± 0.04 L CH4/g VSfed for pretreated stillage, corresponding with an increase in methane yield of about 15%. However, the reactors showed unstable performance with the highest investigated OLRs and shortest HRTs. Under optimal conditions, the conversion efficiencies of COD, cellulose, hemicellulose, and lignin were 88, 95, 97, and 59% for pretreated CWS, and 86, 94, 95, and 51% for untreated CWS, respectively. Microbial community analysis showed that Proteiniphilum, MBA03, and Acetomicrobium were the dominant genera in the digestate and were likely responsible for the conversion of proteins and volatile fatty acids in CWS.

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推进玉米全秸秆嗜热厌氧消化：木质纤维素分解和微生物群落特征描述

将玉米粒转化为生物乙醇是一种不断扩大的可持续燃料生产方式，但同时也会产生大量的副产品，如玉米全渣。在本研究中，使用半连续嗜热反应器评估了高级湿氧化和蒸汽爆炸（AWOEx）预处理对玉米全渣（CWS）的沼气生产和木质纤维素分解的影响。结果表明，在有机负荷率（OLR）为 1.12 ± 0.03 kg VS/m3 天和水力停留时间（HRT）为 30 天的条件下，对玉米全渣进行消化是可行的，未经处理的玉米全渣的甲烷产量为 0.75 ± 0.05 L CH4/g，而经过预处理的玉米全渣的甲烷产量为 0.86 ± 0.04 L CH4/g，甲烷产量增加了约 15%。然而，这些反应器的性能并不稳定，调查的 OLRs 最高，HRTs 最短。在最佳条件下，经预处理的 CWS 的 COD、纤维素、半纤维素和木质素转化率分别为 88%、95%、97% 和 59%，而未经处理的 CWS 的转化率分别为 86%、94%、95% 和 51%。微生物群落分析表明，Proteiniphilum、MBA03 和 Acetomicrobium 是沼渣中的优势菌属，可能负责转化 CWS 中的蛋白质和挥发性脂肪酸。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Fermentation

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