Molecular Survey of Leishmania Infection of Sand Flies in Karun County, Southwestern Iran

IF 0.6 4区医学 Q4 PARASITOLOGY

Journal of Arthropod-Borne Diseases Pub Date : 2024-06-08 DOI:10.18502/jad.v18i1.15670

Shahrzad Taheri, E. Jahanifard, B. Vazirianzadeh, Reza Arjmand

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引用次数: 0

Abstract

Background: Zoonotic cutaneous leishmaniasis (ZCL) is widely distributed in Iran and around the world. Also, Khuzestan Province is an endemic focus of ZCL. This study aims to investigate the natural infection of sand flies with the Leishmania parasite in Karun County. Methods: Sand flies were collected from Jangiyeh, Qaleh Chanan, Kut-e-Navaser, and Ghazavieh in the spring and summer in the year of 2019, by installing 60 sticky paper traps each time (30 traps outdoors and 30 traps indoors). Two hundred female sand flies with different abdominal conditions (empty, blood-fed, semi-gravid, and gravid) were examined for infection rate using the Nested-PCR method. Results: In this study, seven species of sand flies including Phlebotomus papatasi, Ph. alexandri, Ph. sergenti, Ph. caucasicus, Sergentomyia tiberiadis, Se. sintoni, and Se. antennata were reported from Karun County, with a frequency of 79.64%, 16.96%, 1.07%, 0.18%, 0.36%, 1.61%, and 0.18%, respectively. Only eleven specimens of Ph. papatasi were found to be positive for Leishmania major, with an overall infection rate of 7.8%. The infection of Ph. papatasi was specifically reported in blood-fed, gravid, and semi-gravid specimens, with infection rates of 17.02%, 4.35%, and 14.29%, respectively. Conclusion: In this study, the infection of L. major from Ph. papatasi was reported. The results can be used in planning the control of ZCL in the study area.

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伊朗西南部卡伦县沙蝇利什曼原虫感染的分子调查

背景：人畜共患病皮肤利什曼病（ZCL）广泛分布于伊朗和世界各地。此外，胡齐斯坦省也是 ZCL 的流行重点。本研究旨在调查卡伦县沙蝇自然感染利什曼原虫的情况。研究方法于2019年春夏两季在Jangiyeh、Qaleh Chanan、Kut-e-Navaser和Ghazavieh收集沙蝇，每次安装60个粘纸诱捕器（室外30个，室内30个）。采用 Nested-PCR 方法检测了 200 只不同腹部状况（空腹、吸血、半受精和受精）的雌性沙蝇的感染率。结果本研究报告了来自喀伦县的 7 种沙蝇，包括 Phlebotomus papatasi、Ph. alexandri、Ph. sergenti、Ph. caucasicus、Sergentomyia tiberiadis、Se. sintoni 和 Se. antennata，感染率分别为 79.64%、16.96%、1.07%、0.18%、0.36%、1.61% 和 0.18%。只有 11 份 Papatasi Ph. 标本对大利什曼原虫呈阳性，总感染率为 7.8%。据报告，Ph.papatasi 的感染主要发生在血饲标本、怀孕标本和半怀孕标本中，感染率分别为 17.02%、4.35% 和 14.29%。结论本研究报告了Ph. papatasi感染大肠杆菌的情况。研究结果可用于规划研究地区的 ZCL 控制工作。

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来源期刊

Journal of Arthropod-Borne Diseases PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-PARASITOLOGY

CiteScore

2.00

自引率

11.10%

发文量

审稿时长

8 weeks

期刊介绍： The journal publishes original research paper, short communication, scientific note, case report, letter to the editor, and review article in English. The scope of papers comprises all aspects of arthropod borne diseases including: ● Systematics ● Vector ecology ● Epidemiology ● Immunology ● Parasitology ● Molecular biology ● Genetics ● Population dynamics ● Toxicology ● Vector control ● Diagnosis and treatment and other related subjects.