Antibacterial and photo dye degradative ability of copper oxide nanoparticles from Pleurotus cystidiosus

Abstract

The present study deals with in vitro experiments for to develop synthesized copper nanoparticles using Pleurotus cystidiosus. Mushroom-based nanoparticles, such as copper nanoparticles have become more significant because of potential biocatalytic properties, including antibacterial activity. The blue-to-brown color shift suggests CuNPs, as confirmed by absorbance maxima at 246 nm in ultraviolet spectroscopy. FTIR spectra reveal the existence of carboxyl, alcohol, and aldehyde carbon; the amine group represents the myco-synthesized copper nanoparticle. Scanning electron microscopy (SEM) nanoparticles show spherical to oval nano aggregates of 100–130 nm in size. The EDX spectrum confirms the presence of Cu at 8.00 keV in myco-synthesized copper nanoparticles. The antimicrobial efficacy of produced copper nanoparticles on human pathogens showed a maximum zone of inhibition against coliform bacteria such as Enterococcus faecalis, Enterobacter aerogenes, and E. coli, followed by a moderate zone of inhibition recorded in Streptococcus mutans. The photocatalytic activity of Congo red dye, Malachite green dye, and Remazol blue dye by copper nanoparticles from Pleurotus cystidiosus was investigated, and the results showed a maximum degradation efficiency of 99.2% in Congo red within 90 minutes. However, moderate degradation was noted in malachite green, and Remazol blue dye has a low degradation efficiency. Thus, myco-synthesized copper nanoparticles from Pleurotus cystidiosus have potent antimicrobial and photocatalytic dye degradation efficacy.