Respons Imun Mencit terhadap Vaksin DNA Virus Demam Babi Afrika A224L dan A276R dengan Enkapsulasi Lipofektamin, Kolesterol dan Polimer

Abstract

A vaccine against African Swine Fever (ASF) is urgently needed to reduce losses for pig farmers throughout the world. The study was aimed to test the immune response of mice to an ASF-DNA vaccine with the A224L and A276R genes with polymer, lipofectamine, and cholesterol adjuvants. Each mouse was vaccinated intramuscularly with 25 μg of plasmid encapsulated with the above adjuvants. Mouse sera were collected at weeks 1, 2, 3 and 4. Antibodies against A224L and A276R were detected by ELISA test using synthetic peptides as antigens. Optical density (OD) values were analysed statistically using the Analysis of Variance test with the SPSS Version 25. The results showed that the OD values of the serum of mice given the ASF-A224L and A276R DNA vaccines with polymer adjuvants (0.149), lipofectamine (0.080), and cholesterol (0.058), as well as control mice (0.020) differed significantly (p= 0.000), while the time of serum collection had no significant effect (p=0.517). The conclusion is that the OD values of mice sera after DNA-ASF-A224L and A276R encapsulated with polymer, lipofectamine and cholesterol were statistically significant, while sampling time did not show any statistically difference. Further studies are needed to improve the adjuvant mixing protocol with larger quantities of plasmids.Keywords:mice; DNA vaccine; ASF; polymer; ELISA