Synthesis Chitosan nanoparticles from Animal Byproducts (Shrimp Shells) Characterization, Physical Properties and Toxicity of Polymeric Nanoparticles in vivo

Kufa Journal for Agricultural Sciences Pub Date : 2024-06-11 DOI:10.36077/kjas/2024/v16i2.10792

Kadhim H. Abdul Sayed, Alaa G. Al-Hasimi, K. M. Ziadan

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Abstract

The study included the extraction of Chitin (Ch) from shrimp shells and preparation of Chitin nanoparticles (NCh) using acid hydrolysis and ultrasound method. In addition, the preparation of chitosan (Chs) and Chitosan nanoparticles (NChs) by Deacetylation method, then we study some of the characteristics, including yield, diameter by Size Analyzer, Field Emission Scanning Electron Microscopy (FESEM), Fourier transform infrared (FTIR) spectroscopy, X-Ray Diffractometer (X-RD), Viscosity, Water Binding Capacity (WBC) and Fat Binding Capacity (FBC). The results showed, (a) decreased the percentage of yield of NChs to 8.20%, (b) the effective diameter of the natural chitosan using a Size Analyzer and was 28121.5 nm, (c) FESEM analysis revealed that the NChs displayed a nanoscale structure with diameters ranged from 20.93 - 30.20 nm (d) the degree of deacetylation (DD%) using FT-IR spectroscopy device, of NChs was (80.21%) compared to Chs 71.45%, and in addition, from the X-ray spectrum, two peaks in spectra of (Ch, NCh) and (Chs, NChs) were observed at the diffraction angles (2θ) which are (10.70-10.99) and (19.99-20.37). Results show increasing the crystallization coefficient of NCh and NChs, while viscosity, WBC and FBC of NChs decreased compared to Chs. The NChs did not show any toxic effect on human blood cells at concentrations of 100-1000 µg. ml-1.

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从动物副产品（虾壳）中合成壳聚糖纳米粒子高分子纳米粒子的特性、物理特性和体内毒性

该研究包括从虾壳中提取甲壳素（Ch），并采用酸水解和超声法制备甲壳素纳米颗粒（NCh）。此外，还采用去乙酰化法制备了壳聚糖（Chs）和壳聚糖纳米颗粒（NChs），然后研究了它们的一些特性，包括产率、粒径（粒度分析仪）、场发射扫描电子显微镜（FESEM）、傅立叶变换红外光谱（FTIR）、X 射线衍射仪（X-RD）、粘度、水结合力（WBC）和脂肪结合力（FBC）。结果表明：(a) NChs 的产率降低到 8.20%；(b) 使用粒度分析仪测定的天然壳聚糖的有效直径为 28121.5 nm；(c) 原子力场发射光谱分析表明，NChs 显示出纳米级结构，直径范围为 20.93 - 30.20 nm；(d) 天然壳聚糖的粘度降低到 0.5%。20 nm (d) 利用傅立叶变换红外光谱仪测定，NChs 的脱乙酰度（DD%）为 80.21%，而 Chs 为 71.45%，此外，从 X 射线光谱中可以观察到（Ch，NCh）和（Chs，NChs）光谱在衍射角（2θ）处有两个峰，分别为（10.70-10.99）和（19.99-20.37）。结果表明，与 Chs 相比，NCh 和 NChs 的结晶系数增大，而 NChs 的粘度、白细胞计数和全血细胞计数降低。在浓度为 100-1000 µg ml-1 时，NCh 和 NChs 对人血细胞没有任何毒性作用。

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来源期刊

Kufa Journal for Agricultural Sciences

CiteScore

0.20

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0.00%

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