In vitro bioaccessibility and antioxidant properties of unicorn leatherjacket fish (Aluterus monoceros) skin collagen peptides prepared using crude collagenase enzyme isolated from fish fins

Lakshmanan Vinoth Kumar, Robinson Jeyashakila, Sarojini A., Vignaesh Dhanabalan, Marimuthu Manivannan
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Abstract

In this study unicorn leatherjacket (Aluterus monoceros) fish skin collagen was hydrolyzed with the crude collagenase extracted from the fish fins discarded as by-product at three different temperature viz. 50C (CP-5), 250C (CP-25) and 500C (CP-50) to obtain collagen peptide of three molecular mass fraction viz., <30kDa, <10kDa and <3kDa by ultra-filtration using TFF system. The collagenase extracted from fins had a MW of 29kDa and hydrolyzed skin collagen to molecular masses <24kDa efficiently with a DH ranging from 6.6-7.6%. CP-5 peptides with <3kDa showed the maximum antioxidant activity. DPPH and hydroxy radical scavenging activities were good with 70% and 68%, while metal chelating ability was 33% and reducing power was 0.3315. In vitro gastro-intestinal digestion study indicated that after pepsin digestion protein was more in CP-5 than CP-25 and CP-50. The rate of peptide absorption of the CP was significantly high in <3kDa CP-5 (32.56%), followed by CP-25 (32.43%) and CP-50 (32.35%) after gastric and pancreatin digestion. CP-5 of <3kDa having better antioxidative activities of collagen peptides in in vitro gastro-intestinal digests than CP-50 of <3kDa. The study thus indicated that CP with good antioxidant activity shall be produced by hydrolysis the skin at 50C, rather than at 500C.
利用从鱼鳍中分离的粗胶原酶制备独角革囊鱼(Aluterus monoceros)皮肤胶原蛋白肽的体外生物可及性和抗氧化特性
本研究利用从鱼鳍中提取的粗胶原蛋白酶,在50℃(CP-5)、250℃(CP-25)和500℃(CP-50)三种不同温度下,通过TFF系统进行超滤,水解麒麟鱼(Aluterus monoceros)鱼皮胶原蛋白,得到三种分子质量的胶原蛋白肽,即<30kDa、<10kDa和<3kDa。从鱼鳍中提取的胶原酶的分子量为 29kDa,能有效地将皮肤胶原蛋白水解为分子质量 <24kDa 的肽,其 DH 值在 6.6-7.6% 之间。分子量小于 3kDa 的 CP-5 肽的抗氧化活性最高。DPPH 和羟基自由基清除活性分别为 70% 和 68%,金属螯合能力为 33%,还原力为 0.3315。体外胃肠消化研究表明,胃蛋白酶消化后,CP-5 中的蛋白质含量高于 CP-25 和 CP-50。经胃消化和胰蛋白酶消化后,肽的吸收率在<3kDa 的 CP-5 中明显较高(32.56%),其次是 CP-25 (32.43%)和 CP-50 (32.35%)。在体外胃肠消化中,<3kDa 的 CP-5 比<3kDa 的 CP-50 具有更好的胶原蛋白肽抗氧化活性。因此,该研究表明,在 50C 而不是 500C 下水解皮肤可产生具有良好抗氧化活性的 CP。
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