Investigation of some nucleoli traits in interphase leukocytes of two rabbit breeds and their hybrid

IF 0.4 Q4 AGRICULTURE, MULTIDISCIPLINARY

Agricultural Science and Practice Pub Date : 2024-06-13 DOI:10.15407/agrisp11.01.070

V. Dsitsiuk, O. Boiko, O. Honchar, O. Havrysh, O. Guzevatiy

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Abstract

Aim. The aim was to study the differences in three traits of nucleoli in interphase leukocytes of two rab- bit breeds and a hybrid thereof. Methods. Doe rabbits, 4-month -old, from Ukrainian breed Poltavske Sriblo (n = 30), Californian (n = 25), and their hybrid (n = 21) were used in the experiment. The nucleoli in intact blood leukocytes were stained using silver staining according to Howell and Black (1980). Stained cells were observed, and their nucleoli counted in light microscopy at 10×100 oil immersion magnification. 200 leukocytes from each animal were analyzed. The activity of nucleoli was evaluated by the following parameters: the aver- age number of nucleoli in the nucleus (nNO), the total area of nucleoli in the nucleus (ΣSNO, mcm 2), the share of nucleolus area in the lymphocyte nucleus area (shΣS NO, %). Statistical analysis was conducted using the STATISTICA software package (2020). Results. The average number of nucleoli per cell varied from – 1.70 ± 0.08 in Californian rabbits to 5.90 ± 0.29 in hybrid animals. A statistically significant difference (p < 0.05) was found between the experimental groups of purebred and hybrid rabbits. The variation coefficient for the index of the average number of nucleoli per cell was on the average level of variability: 20.58 % for the rabbits of Poltavske sriblo breed, 19.50 % for Californian rabbits, and 16.49 % for hybrid ones. The total area of nucleoli in the cells of all the investigated animals varied from 5 μm2 in one Californian rabbit to 12 μm2 in animals of hybrid origin. The share of the nucleolus area in the nucleus area for rabbits of Poltavske sriblo, Californian, and hybrid breeds was 26.10 ± 1.80 %, 24.30 ± 1.62 and 29.40 ± 2.50, respectively. Conclusions. Polymorphism was observed for three nucleolar parameters after silver staining of interphase leukocytes of rabbits of Poltavske sriblo, Californian breed and their hybrid. This concerned 1) the average number of nucleoli (p < 0.05); 2) the total area of nucleoli (p < 0.05); 3) the average share of the nucleolar area in the nucleus area (p < 0.05). The results of our comparative analysis of the investigated nucleolar activity parameters suggest a higher activity of nucleoli in the animals of hybrid origin. In the future, the results of such studies may be used to assess the potential ability of animals to implement productive traits.

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对两个兔种及其杂交种相间白细胞中某些核小体特征的研究

研究目的目的是研究两个狂犬品种和一个杂交品种间期白细胞核小体三个特征的差异。方法。实验使用了乌克兰品种 Poltavske Sriblo（n = 30）、加利福尼亚（n = 25）及其杂交品种（n = 21）的 4 月龄母兔。根据 Howell 和 Black（1980 年）的方法，用银染色法对完整血白细胞的核小体进行染色。观察染色细胞，并在光镜下以 10×100 的油浸放大倍率计数核小体。对每只动物的 200 个白细胞进行分析。核小体的活性通过以下参数进行评估：核内核小体的平均年龄数（nNO）、核内核小体的总面积（ΣSNO，mcm 2）、核小体面积占淋巴细胞核面积的比例（shΣS NO，%）。统计分析使用 STATISTICA 软件包（2020）进行。结果每个细胞的平均核小体数量从加州兔的 - 1.70 ±0.08 到杂交动物的 5.90 ± 0.29 不等。纯种兔和杂交兔实验组之间的差异有统计学意义（P < 0.05）。每个细胞平均核小体数量指标的变异系数处于平均变异水平：波尔塔夫斯克氏兔为 20.58%，加利福尼亚兔为 19.50%，杂交兔为 16.49%。所有被调查动物细胞中的核小体总面积各不相同，加州兔为 5 μm2，杂交兔为 12 μm2。Poltavske sriblo 兔、加利福尼亚兔和杂交兔的核小体面积占细胞核面积的比例分别为 26.10 ± 1.80 %、24.30 ± 1.62 % 和 29.40 ± 2.50 %。结论对波尔塔夫斯克里布洛兔、加利福尼亚兔和杂交兔的间期白细胞进行银染色后，观察到三个核小体参数存在多态性。这涉及 1) 核小体的平均数量 (p < 0.05)；2) 核小体的总面积 (p < 0.05)；3) 核小体面积占核面积的平均比例 (p < 0.05)。我们对所研究的核小体活性参数进行比较分析的结果表明，杂交种动物的核小体活性较高。未来，此类研究结果可用于评估动物实现生产性状的潜在能力。

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Agricultural Science and Practice AGRICULTURE, MULTIDISCIPLINARY-

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