Raffinose Ameliorates DSS-Induced Colitis in Mice by Modulating Gut Microbiota and Targeting the Inflammatory TLR4–MyD88–NF-κB Signaling Pathway

Foods Pub Date : 2024-06-13 DOI:10.3390/foods13121849
Peng Zhang, Yuyang Xue, Zhengyu Cao, Yaya Guo, Xiaotong Pang, Cheng Chen, Wenju Zhang
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Abstract

This study aimed to explore the protective effects of raffinose (Raf) against inflammatory bowel disease in mice with colitis. Mice were administered 100, 200, or 400 mg/kg Raf for 21 d, followed by drinking-water containing 3% dextran sulfate sodium salt (DSS) for 3 d. Thereafter, the phenotype, pathological lesions in the colon, cytokines levels, and gut microbiota were evaluated. Treatment with Raf reduced the severity of the pathological changes in the colon, mitigating the reduction in colon length. Following Raf intervention, serum levels of inflammatory cytokines (IL-2, IL-6, IL-1β, and TNF-α) tended to return to normal. These results suggest that the anti-inflammatory effects of Raf are associated with a reduction in TLR4–MyD88–NF-κB pathway expression in mouse colonic tissues. Analysis of gut microbiota abundance and its correlation with colitis parameters revealed that DSS-induced dysbiosis was partially mitigated by Raf. In conclusion, Raf exerts a protective effect in colitis by modulating the gut microbiota and TLR4-MyD88-NF-κB pathway.
棉子糖通过调节肠道微生物群和靶向炎症性 TLR4-MyD88-NF-κB 信号通路改善小鼠 DSS 诱发的结肠炎
本研究旨在探讨棉子糖 (Raf) 对结肠炎小鼠炎症性肠病的保护作用。给小鼠注射 100、200 或 400 毫克/千克 Raf 达 21 天,然后用含 3% 右旋糖酐硫酸钠盐(DSS)的饮用水治疗 3 天,之后评估小鼠的表型、结肠病变、细胞因子水平和肠道微生物群。Raf 治疗减轻了结肠病理变化的严重程度,缓解了结肠长度的减少。Raf干预后,血清中的炎性细胞因子(IL-2、IL-6、IL-1β和TNF-α)水平趋于恢复正常。这些结果表明,Raf 的抗炎作用与小鼠结肠组织中 TLR4-MyD88-NF-κB 通路表达的减少有关。肠道微生物群丰度及其与结肠炎参数的相关性分析表明,Raf 可部分缓解 DSS 诱导的菌群失调。总之,Raf 通过调节肠道微生物群和 TLR4-MyD88-NF-κB 通路对结肠炎具有保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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