The Important Role of Denitrifying Exoelectrogens in Single-Chamber Microbial Fuel Cells after Nitrate Exposure

IF 2.5 4区工程技术 Q3 CHEMISTRY, ANALYTICAL

Separations Pub Date : 2024-06-14 DOI:10.3390/separations11060187

Xiaojun Jin, Wenyi Wang, Zhuo Yan, Dake Xu

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引用次数: 0

Abstract

Wastewater treatment using microbial fuel cells (MFCs) is a potentially useful technology due to its low cost, environmental friendliness, and low sludge production. In this study, a single-chambered air cathode MFC (SCMFC) was developed and investigated regarding its performance and microbial community evolution following nitrate exposure. During long-term operation, diverse denitrifiers accumulated on the electrodes to form a denitrifying MFC (DNMFC) with stable activity for nitrate reduction. The DNMFC presented considerably higher electroactivity, stability, and denitrification rates than the SCMFC. Though energy recovery decreased in the DNMFC by partial organics utilized for heterotrophic denitrification, the electron transfer efficiency increased. Geobacter as the absolutely dominant genus in the SCMFC anode was eliminated and replaced by Azonexus and Pseudomonas in the DNMFC. Furthermore, the biomass of Pseudomonas (151.0 ng/μL) in the DNMFC cathode was five-fold higher than that in the SCMFC, although the bacterial community compositions were quite similar. The DNMFC with highly abundant Pseudomonas exhibited much better performance in terms of electrochemical activity and nitrate removal. The evolution process of functional bacteria from the SCMFC to the DNMFC comprehensively reveals the significant role of denitrifying electroactive bacteria in a bioelectrochemical system for nitrogen-containing wastewater treatment.

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单腔微生物燃料电池暴露于硝酸盐后脱硝外源的重要作用

使用微生物燃料电池（MFC）处理废水是一项潜在的有用技术，因为它成本低、环保且污泥产量低。本研究开发了一种单室空气阴极 MFC（SCMFC），并对其性能和硝酸盐暴露后的微生物群落演化进行了研究。在长期运行过程中，电极上积累了多种反硝化菌，形成了具有稳定硝酸盐还原活性的反硝化 MFC（DNMFC）。DNMFC 的电活性、稳定性和反硝化率都大大高于 SCMFC。虽然 DNMFC 的能量回收率因利用部分有机物进行异养反硝化而有所降低，但电子传递效率却有所提高。在 SCMFC 阳极中占绝对优势的革兰氏菌属在 DNMFC 中被淘汰，取而代之的是 Azonexus 和假单胞菌。此外，尽管细菌群落组成非常相似，但 DNMFC 阴极中假单胞菌的生物量（151.0 ng/μL）比 SCMFC 高出五倍。含有大量假单胞菌的 DNMFC 在电化学活性和硝酸盐去除方面表现出更好的性能。功能菌从 SCMFC 到 DNMFC 的进化过程全面揭示了反硝化电活性菌在含氮废水处理生物电化学系统中的重要作用。

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来源期刊

Separations Chemistry-Analytical Chemistry

CiteScore

3.00

自引率

15.40%

发文量

342

审稿时长

12 weeks

期刊介绍： Separations (formerly Chromatography, ISSN 2227-9075, CODEN: CHROBV) provides an advanced forum for separation and purification science and technology in all areas of chemical, biological and physical science. It publishes reviews, regular research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, unique features of this journal: Manuscripts regarding research proposals and research ideas will be particularly welcomed. Electronic files and software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. Manuscripts concerning summaries and surveys on research cooperation and projects (that are funded by national governments) to give information for a broad field of users. The scope of the journal includes but is not limited to: Theory and methodology (theory of separation methods, sample preparation, instrumental and column developments, new separation methodologies, etc.) Equipment and techniques, novel hyphenated analytical solutions (significantly extended by their combination with spectroscopic methods and in particular, mass spectrometry) Novel analysis approaches and applications to solve analytical challenges which utilize chromatographic separations as a key step in the overall solution Computational modelling of separations for the purpose of fundamental understanding and/or chromatographic optimization