Development of an efficient process to extract C-phycocyanin from marine cyanobacterial strains and evaluation of its bioactive potential

IF 2.8 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology Pub Date : 2024-06-14 DOI:10.1002/jctb.7693

Mahammed Ilyas Khazi, Zeliha Demirel, Fakhra Liaqat, Rengin Eltem, Meltem Conk Dalay

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引用次数: 0

Abstract

BACKGROUND

Phycocyanin (PC), a natural blue photosynthetic pigment found in cyanobacteria, rhodophyta and cryptophyta has numerous applications in biotechnology and the food industry. The phycocyanin extraction method has a significant impact on its bioactivities, purity index, cost and potential applications; thus, the development of an efficient extraction strategy continues to be a primary research goal.

RESULTS

This study evaluated the efficacy of different methods for the extraction of cyanobacterial phycocyanin (C-PC) from three marine cyanobacterial isolates, namely Arthrospira platensis, Phormidium sp. and Pseudoscillatoria sp. wet biomass. The pretreatment step of soaking biomass in extraction buffer for 60 min, followed by a combination of freeze–thaw (one cycle; 2 h) and sonication (2 min) treatment was found to be the most effective extraction strategy, producing the highest C-PC yield (16.19 ± 0.34%, 15.89 ± 0.61% and 15.56 ± 0.69% dry cell weight for strains A. platensis, Phormidium sp. and Pseudoscillatoria sp., respectively). Furthermore, a biomass-to-solvent ratio of 0.02 g mL⁻¹ was optimal to attain food-grade purity ((A₆₂₀/A₂₈₀) ≥ 0.7). Bioactivity evaluation of crude and purified C-PC exhibited its potential as antioxidant compounds and as an antimicrobial agent with significant inhibitory effects against various pathogenic microorganisms.

CONCLUSION

The study proposed an efficient and cost-effective C-PC extraction process for cyanobacteria and highlighted the potential of C-PC as a valuable bioactive compound with various applications. © 2024 Society of Chemical Industry (SCI).

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开发从海洋蓝藻菌株中提取 C-phycocyanin 的高效工艺并评估其生物活性潜力

藻蓝蛋白（PC）是一种存在于蓝藻、红藻和隐藻中的天然蓝色光合色素，在生物技术和食品工业中有着广泛的应用。本研究评估了从三种海洋蓝藻分离物（即 Arthrospira platensis、Phormidium sp.和 Pseudoscillatoria sp.）湿生物质中提取蓝藻藻蓝蛋白（C-PC）的不同方法的功效。研究发现，将生物质在萃取缓冲液中浸泡 60 分钟，然后进行冻融（一个周期；2 小时）和超声（2 分钟）处理是最有效的萃取策略，可产生最高的 C-PC 产量（A. platensis 菌株、Phormidium sp.菌株和 Pseudoscillatoria sp.菌株的干细胞重量分别为 16.19 ± 0.34%、15.89 ± 0.61% 和 15.56 ± 0.69%）。此外，要达到食品级纯度（(A620/A280) ≥ 0.7），生物质与溶剂的比率为 0.02 g mL-1 是最佳的。该研究提出了一种高效、经济的蓝藻 C-PC 提取工艺，并强调了 C-PC 作为一种有价值的生物活性化合物的各种应用潜力。© 2024 化学工业学会（SCI）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of chemical technology and biotechnology 工程技术-工程：化工

CiteScore

7.00

自引率

5.90%

发文量

268

审稿时长

1.7 months

期刊介绍： Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.