Key genes and metabolites that regulate wool fibre diameter identified by combined transcriptome and metabolome analysis

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Lin Yue , Zengkui Lu , Tingting Guo , Jianbin Liu , Bohui Yang , Chao Yuan
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Abstract

Background

Fibre diameter is an important economic trait of wool fibre. As the fibre diameter decreases, the economic value of wool increases. Therefore, understanding the mechanism of wool fibre diameter regulation is important in improving the value of wool.

Results

In this study, we used non-targeted metabolome and reference transcriptome data to detect differences in metabolites and genes in groups of Alpine Merino sheep with different wool fibre diameter gradients, and integrated metabolome and transcriptome data to identify key genes and metabolites that regulate wool fibre diameter. We found 464 differentially abundant metabolites (DAMs) and 901 differentially expressed genes (DEGs) in four comparisons of groups with different wool fibre diameters. Approximately 25% of the differentially abundant metabolites were lipid and lipid-like molecules. These molecules were predicted to be associated with skin development and keratin filament by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses. Key genes, including COL5A2, COL5A3, CREB3L4, COL1A1, and SFRP4, were identified by gene set enrichment analysis.

Conclusions

Key genes regulating wool fibre diameter were identified, the effects of lipid molecules on wool performance were investigated, and potential synergies between genes and metabolites were postulated, providing a theoretical framework for fine wool sheep breeding.

通过联合转录组和代谢组分析,确定调控羊毛纤维直径的关键基因和代谢物。
背景:纤维直径是羊毛纤维的一个重要经济特征。纤维直径越小,羊毛的经济价值越高。因此,了解羊毛纤维直径的调节机制对于提高羊毛的价值非常重要:在这项研究中,我们利用非靶向代谢组和参考转录组数据检测了不同羊毛纤维直径梯度的阿尔卑斯美利奴羊群体中代谢物和基因的差异,并整合代谢组和转录组数据确定了调控羊毛纤维直径的关键基因和代谢物。我们在不同羊毛纤维直径组别的四次比较中发现了 464 种差异丰度代谢物(DAMs)和 901 个差异表达基因(DEGs)。大约 25% 的差异丰度代谢物是脂质和类脂质分子。通过基因本体(GO)和京都基因和基因组百科全书(KEGG)功能富集分析,预测这些分子与皮肤发育和角蛋白丝有关。通过基因组富集分析,确定了包括 COL5A2、COL5A3、CREB3L4、COL1A1 和 SFRP4 在内的关键基因:结论:确定了调控羊毛纤维直径的关键基因,研究了脂质分子对羊毛性能的影响,推测了基因与代谢物之间潜在的协同作用,为细毛羊育种提供了理论框架。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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