Single-molecule fluorescence imaging of DNA maintenance protein binding dynamics and activities on extended DNA

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2024-06-15 DOI:10.1016/j.sbi.2024.102863

Elizabeth Marie Irvin , Hong Wang

引用次数: 0

Abstract

Defining the molecular mechanisms by which genome maintenance proteins dynamically associate with and process DNA is essential to understand the potential avenues by which these stabilizing mechanisms are disrupted. Single-molecule fluorescence imaging (SMFI) of protein dynamics on extended DNA has greatly expanded our ability to accomplish this, as it captures singular biomolecular interactions – in all their complexity and diversity – without relying on ensemble-averaging of bulk protein activity as most traditional biochemical techniques must do. In this review, we discuss how SMFI studies with extended DNA have substantially contributed to genome stability research over the past two years.

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扩展 DNA 上 DNA 维护蛋白结合动态和活动的单分子荧光成像

要了解这些稳定机制被破坏的潜在途径，就必须确定基因组维护蛋白与 DNA 动态关联和处理 DNA 的分子机制。对扩展 DNA 上蛋白质动态的单分子荧光成像（SMFI）极大地拓展了我们实现这一目标的能力，因为它能捕捉单个生物分子的相互作用--其复杂性和多样性--而不像大多数传统生化技术那样必须依赖于大量蛋白质活性的集合平均。在这篇综述中，我们将讨论在过去两年中，利用扩展 DNA 进行的 SMFI 研究是如何为基因组稳定性研究做出重大贡献的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation