Toxicological effects of nodularin on the reproductive endocrine system of female zebrafish (Danio rerio)

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-06-10 DOI:10.1016/j.aquatox.2024.107000

Chayan Biswas, Madhuchhanda Adhikari, Kousik Pramanick

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Abstract

Nodularin is a potent cyanotoxin that has been detected in aquatic environments as well as in the body of aquatic organisms throughout the world, but its effects on the reproductive system are yet to be explored. The present study investigated the toxic effects of environmentally relevant concentrations of nodularin on the reproductive endocrine system of female zebrafish (Danio rerio). After exposure to nodularin for 14 days, decreased gonadosomatic Index (GSI), germinal vesicle breakdown (GVBD), and decreased level of follicle-stimulating hormone (FSH), luteinizing hormone (LH), 17β-estradiol (E2) level and increased testosterone (T) content in female zebrafish suggested that nodularin may disrupt both oocyte growth and maturation. In support of this data, alteration in different marker gene expression on the hypothalamic-pituitary-gonadal-liver (HPGL) axis was observed. Transcriptional levels of genes related to steroidogenesis including cytochrome P450 aromatase (cyp19a1a) in the ovary and primary vitellogenin genes (vtg1, vtg2, and vtg3) in the liver were down-regulated and marker genes for oxidative stress (sod, cat, and gpx) were up-regulated on HPGL axis. These findings revealed for the first time that nodularin is a potent endocrine-disrupting compound posing oxidative stress and causes reproductive endocrine toxicity in female zebrafish, emphasizing the importance of assessing its environmental risks.

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点头素对雌性斑马鱼（Danio rerio）生殖内分泌系统的毒理学影响。

结核霉素是一种强效的蓝藻毒素，在世界各地的水生环境和水生生物体内均有发现，但其对生殖系统的影响尚待探索。本研究调查了环境相关浓度的点头素对雌性斑马鱼（Danio rerio）生殖内分泌系统的毒性影响。雌性斑马鱼暴露于点头素 14 天后，性腺指数（GSI）下降，生殖囊破裂（GVBD），卵泡刺激素（FSH）、黄体生成素（LH）、17β-雌二醇（E2）水平下降，睾酮（T）含量增加，这表明点头素可能会破坏卵母细胞的生长和成熟。为支持这一数据，观察到下丘脑-垂体-性腺-肝脏（HPGL）轴上不同标记基因的表达发生了变化。与类固醇生成有关的基因，包括卵巢中的细胞色素 P450 芳香化酶（cyp19a1a）和肝脏中的初级卵黄素基因（vtg1、vtg2 和 vtg3）的转录水平下调，而 HPGL 轴上的氧化应激标记基因（sod、cat 和 gpx）上调。这些发现首次揭示了Nodularin是一种强效内分泌干扰化合物，会对雌性斑马鱼造成氧化应激和生殖内分泌毒性，强调了评估其环境风险的重要性。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.