Identification and distribution of some medico-veterinary important pathogens in muscid flies in two geographical regions of Türkiye

IF 1.6 3区农林科学 Q2 ENTOMOLOGY

Medical and Veterinary Entomology Pub Date : 2024-06-12 DOI:10.1111/mve.12734

Nuri Ercan, Alparslan Yildirim, Onder Duzlu, Fahriye Ercan, Gamze Yetismis, Gokmen Zafer Pekmezci, Abdullah Inci

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Abstract

Some dipteran flies play an important role in the transmission of pathogens such as viruses, bacteria, fungi, protozoan and metazoan parasites in humans and other animals. Despite this importance, knowledge of the prevalence and molecular characteristics of some pathogens in flies is limited, and no data are available for Türkiye. In this study, we investigated the possible vector role of muscid fly species for the transmission of Enterocytozoon bieneusi Desportes (Chytridiopsida: Enterocytozoonidae), Encephalitozoon spp., Coxiella burnetii Derrick (Legionellales: Coxiellaceae) and Thelazia spp. using polymerase chain reaction (PCR) and sequence analysis. The flies were trapped in different animal-related places and surroundings from two different geographical regions of Türkiye including Central Anatolia and Middle Black Sea. According to the morphological keys, 850 (85%), 141 (14.1%) and 6 (0.6%) of the total of 1000 fly specimens identified as Musca domestica Linnaeus (Diptera: Muscidae), Stomoxys calcitrans Linnaeus (Diptera: Muscidae) and Musca autumnalis De Geer (Diptera: Muscidae), respectively. The other species including Haematobia irritans Linnaeus (Diptera: Muscidae), Muscina stabulans Fallén (Diptera: Muscidae) and Hydrotaea ignava Harris (Diptera: Muscidae) were each represented by a single specimen. Screening of the pathogens identified E. bieneusi only in M. domestica with a prevalence of 2.4%. Sequence analyses identified three known genotypes, Type IV, BEB6 and BEB8, and one novel genotype named AEUEb of E. bieneusi in M. domestica. Coxiella burnetii was detected in M. domestica and S. calcitrans with prevalences of 2.9% and 2.8%, respectively. The one specimen of H. ignava was also positive for C. burnetii. Encephalitozoon spp. and Thelazia spp. were not found in the examined specimens. Our results contribute to the current knowledge on the vector potential of muscid flies and their possible role in the transmission dynamics of certain pathogens, especially in regions where diseases are prevalent and affect public and animal health.

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图尔基耶两个地理区域的麝香蝇中一些重要的兽医病原体的鉴定和分布。

一些双翅目苍蝇在人类和其他动物体内传播病毒、细菌、真菌、原生动物和元虫寄生虫等病原体方面发挥着重要作用。尽管如此重要，但对苍蝇中某些病原体的流行和分子特征的了解却很有限，土耳其也没有这方面的数据。在这项研究中，我们利用聚合酶链式反应（PCR）和序列分析，研究了麝香蝇物种在传播 Enterocytozoon bieneusi Desportes（蝶形目：Enterocytozoonidae）、Encephalitozoon spp.、Coxxiella burnetii Derrick（军团菌纲：Coxiellaceae）和 Thelazia spp.这些苍蝇是从土耳其两个不同的地理区域（包括安纳托利亚中部和黑海中部）的不同动物相关场所和周围环境中捕获的。根据形态学钥匙，在总共 1000 份苍蝇标本中，分别有 850 份（85%）、141 份（14.1%）和 6 份（0.6%）被鉴定为林尼厄斯家蝇（双翅目：鹟科）、Stomoxys calcitrans Linnaeus（双翅目：鹟科）和 De Geer 秋蝇（双翅目：鹟科）。其他物种包括 Haematobia irritans Linnaeus（双翅目：鹟科）、Muscina stabulans Fallén（双翅目：鹟科）和 Hydrotaea ignava Harris（双翅目：鹟科），每个物种只有一个标本。病原体筛查仅在 M. domestica 中发现了 E. bieneusi，发病率为 2.4%。序列分析确定了三种已知的基因型：IV 型、BEB6 型和 BEB8 型，以及一种名为 AEUEb 的新型基因型。在 M. domestica 和 S. calcitrans 中检测到了烧伤柯西氏菌，感染率分别为 2.9% 和 2.8%。点火蚁的一份标本也对烧伤蜱呈阳性反应。在检查的标本中没有发现脑线虫属（Encephalitozoon spp.）和蝶形花属（Thelazia spp.）。我们的研究结果有助于进一步了解蕈蚊蝇的媒介潜力及其在某些病原体传播动态中可能扮演的角色，尤其是在疾病流行并影响公共和动物健康的地区。

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来源期刊

Medical and Veterinary Entomology 农林科学-昆虫学

CiteScore

3.70

自引率

5.30%

发文量

审稿时长

12-24 weeks

期刊介绍： Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of: -epidemiology and transmission of vector-borne pathogens changes in vector distribution that have impact on the pathogen transmission- arthropod behaviour and ecology- novel, field evaluated, approaches to biological and chemical control methods- host arthropod interactions. Please note that we do not consider submissions in forensic entomology.