Unveiling the role of AGT in lipid metabolism and regulated cell death in colon cancer

IF 4.8 2区医学 Q1 Biochemistry, Genetics and Molecular Biology

Neoplasia Pub Date : 2024-06-07 DOI:10.1016/j.neo.2024.101009

Mengdi Wu , Yuyang Zhou , Dongsheng Pei , Shoucui Gao

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引用次数: 0

Abstract

Background

Lipid metabolism and regulated cell death (RCD) play a role in the remodeling of tumor immune microenvironment and regulation of cancer progression. Since the underlying immune mechanisms of colon cancer remain elusive, this study aims to identify potential therapeutic target genes.

Methods

Differential genes related to lipid metabolism and RCD in COAD patients were identified using R language and online tools. Based on the expression of genes, two groups were classified using consensus clustering. CIBERSORT and ssGSEA were used to detect immune infiltration in both groups. Prognostic signature genes for colon cancer were screened using machine learning algorithms. KEGG, GO and GSEA for gene pathway enrichment. In addition, interacting genes in the immune module were obtained using a weighted gene co-expression network (WGCNA). Finally, expression and mutation of key in colon cancer genes were detected using TIMER, HPR, cBioPortal website and qPCR.

Results

The consensus clustering analysis revealed that 231 relevant differential genes were highly associated with immune infiltration. A series of machine learning and website analyses identified AGT as a hub gene linked to lipid metabolism and regulated cell death, which is overexpressed in colon cancer.

Conclusion

AGT, as a signature gene of lipid metabolism and regulated cell death, plays a critical role in the development of COAD and is associated with tumor immune infiltration.

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揭示 AGT 在结肠癌脂质代谢和调节细胞死亡中的作用

背景脂质代谢和调节性细胞死亡（RCD）在重塑肿瘤免疫微环境和调控癌症进展中发挥作用。方法使用 R 语言和在线工具鉴定 COAD 患者中与脂质代谢和 RCD 相关的差异基因。根据基因的表达情况，采用共识聚类将其分为两组。CIBERSORT和ssGSEA用于检测两组中的免疫浸润。使用机器学习算法筛选结肠癌的预后特征基因。利用 KEGG、GO 和 GSEA 进行基因通路富集。此外，还利用加权基因共表达网络（WGCNA）获得了免疫模块中的相互作用基因。最后，利用 TIMER、HPR、cBioPortal 网站和 qPCR 对结肠癌关键基因的表达和突变进行了检测。结论AGT作为脂质代谢和细胞死亡调控的标志基因，在COAD的发生发展中起着关键作用，并与肿瘤免疫浸润相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neoplasia 医学-肿瘤学

CiteScore

9.20

自引率

2.10%

发文量

审稿时长

26 days

期刊介绍： Neoplasia publishes the results of novel investigations in all areas of oncology research. The title Neoplasia was chosen to convey the journal’s breadth, which encompasses the traditional disciplines of cancer research as well as emerging fields and interdisciplinary investigations. Neoplasia is interested in studies describing new molecular and genetic findings relating to the neoplastic phenotype and in laboratory and clinical studies demonstrating creative applications of advances in the basic sciences to risk assessment, prognostic indications, detection, diagnosis, and treatment. In addition to regular Research Reports, Neoplasia also publishes Reviews and Meeting Reports. Neoplasia is committed to ensuring a thorough, fair, and rapid review and publication schedule to further its mission of serving both the scientific and clinical communities by disseminating important data and ideas in cancer research.