Effect of atractylenolide III on zearalenone-induced Snail1-mediated epithelial-mesenchymal transition in porcine intestinal epithelium.

IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Na Yeon Kim, Myoung Ok Kim, Sangsu Shin, Woo-Sung Kwon, Bomi Kim, Joon Yeop Lee, Sang In Lee
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引用次数: 0

Abstract

Background: The intestinal epithelium performs essential physiological functions, such as nutrient absorption, and acts as a barrier to prevent the entry of harmful substances. Mycotoxins are prevalent contaminants found in animal feed that exert harmful effects on the health of livestock. Zearalenone (ZEA) is produced by the Fusarium genus and induces gastrointestinal dysfunction and disrupts the health and immune system of animals. Here, we evaluated the molecular mechanisms that regulate the effects of ZEA on the porcine intestinal epithelium.

Results: Treatment of IPEC-J2 cells with ZEA decreased the expression of E-cadherin and increased the expression of Snai1 and Vimentin, which induced Snail1-mediated epithelial-to-mesenchymal transition (EMT). In addition, ZEA induces Snail-mediated EMT through the activation of TGF-β signaling. The treatment of IPEC-J2 cells with atractylenolide III, which were exposed to ZEA, alleviated EMT.

Conclusions: Our findings provide insights into the molecular mechanisms of ZEA toxicity in porcine intestinal epithelial cells and ways to mitigate it.

苍术内酯 III 对玉米赤霉烯酮诱导的猪肠上皮细胞蜗牛1介导的上皮-间质转化的影响
背景:肠道上皮细胞发挥着重要的生理功能,如营养吸收,同时也是防止有害物质进入的屏障。霉菌毒素是动物饲料中普遍存在的污染物,会对牲畜的健康产生有害影响。玉米赤霉烯酮(ZEA)由镰刀菌属产生,可诱发胃肠功能紊乱,破坏动物的健康和免疫系统。在此,我们评估了调节玉米赤霉烯酮对猪肠上皮细胞影响的分子机制:结果:用 ZEA 处理 IPEC-J2 细胞会降低 E-cadherin 的表达,增加 Snai1 和 Vimentin 的表达,从而诱导 Snail1 介导的上皮细胞向间质转化(EMT)。此外,ZEA 还通过激活 TGF-β 信号传导诱导 Snail 介导的 EMT。用苍术内酯 III 处理暴露于 ZEA 的 IPEC-J2 细胞可减轻 EMT:我们的研究结果有助于深入了解猪肠上皮细胞中 ZEA 毒性的分子机制以及减轻其毒性的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
10.30
自引率
0.00%
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822
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