Abnormalities of Coagulation and Fibrinolysis Assessed by Thromboelastometry in an Endotoxic Shock Model in Piglets Treated with Nitric Oxide and Hydrocortisone.

IF 2.9 4区 医学 Q3 IMMUNOLOGY
Archivum Immunologiae et Therapiae Experimentalis Pub Date : 2024-06-07 eCollection Date: 2024-01-01 DOI:10.2478/aite-2024-0011
Barbara Adamik, Claes Frostell, Barbara Dragan, Urszula Paslawska, Stanislaw Zielinski, Robert Paslawski, Adrian Janiszewski, Marzena Zielinska, Stanislaw Ryniak, Johanna Albert, Waldemar Gozdzik
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引用次数: 0

Abstract

This is an animal model study to investigate changes in hemostasis during endotoxemic shock and to determine whether the combination of inhaled nitric oxide (iNO) + intravenous hydrocortisone had an effect on clot formation and fibrinolysis. iNO selectively decreases pulmonary artery pressure, without affecting cardiac index or systemic vascular resistance; however, the results of studies on the possible consequences of iNO administration on coagulation are inconsistent and require further research. Thirty-four piglets were included. Administering endotoxin caused severe hypodynamic shock. Half of the animals received iNO (30 ppm) + hydrocortisone, starting 3 h after endotoxin infusion and continuing to the end of the study. All animals developed coagulation disorders, manifested by a tendency to hypocoagulation; at the same time, fibrinolysis was impaired. Coagulation and fibrinolysis disorders persisted after endotoxin infusion was discontinued, with worse severity in the animals that died before the study was terminated. Administering iNO + hydrocortisone did not cause further changes in coagulation and fibrinolysis parameters, either during or after the endotoxin challenge, suggesting that potential therapeutic interventions with iNO to lower pulmonary arterial pressure will not affect hemostasis.

使用一氧化氮和氢化可的松治疗内毒素休克模型的仔猪血栓弹力测定法评估凝血和纤维蛋白溶解异常情况
这是一项动物模型研究,旨在探讨内毒素性休克期间止血的变化,并确定吸入一氧化氮(iNO)+静脉注射氢化可的松的组合是否会对血凝块的形成和纤维蛋白溶解产生影响。iNO可选择性地降低肺动脉压,而不会影响心脏指数或全身血管阻力;但是,关于吸入一氧化氮对凝血可能产生的影响的研究结果并不一致,需要进一步研究。本研究共纳入 34 头仔猪。注射内毒素会导致严重的低动力性休克。一半的动物接受了 iNO(30 ppm)+氢化可的松治疗,从注入内毒素后 3 小时开始,一直持续到研究结束。所有动物都出现了凝血功能障碍,表现为低凝倾向;同时,纤溶功能也受到了损害。在停止输注内毒素后,凝血和纤溶紊乱仍持续存在,在研究结束前死亡的动物中,凝血和纤溶紊乱的情况更为严重。无论是在内毒素挑战期间还是之后,给予 iNO + 氢化可的松都不会引起凝血和纤溶参数的进一步变化,这表明使用 iNO 降低肺动脉压的潜在治疗干预措施不会影响止血。
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来源期刊
CiteScore
5.90
自引率
0.00%
发文量
26
审稿时长
>12 weeks
期刊介绍: Archivum Immunologiae et Therapiae Experimentalis (AITE), founded in 1953 by Ludwik Hirszfeld, is a bimonthly, multidisciplinary journal. It publishes reviews and full original papers dealing with immunology, experimental therapy, immunogenetics, transplantation, microbiology, immunochemistry and ethics in science.
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