Screening of lentil genotypes during germination and early growth stages under PEG-induced drought stress

IF 1.7 4区农林科学 Q2 Agricultural and Biological Sciences

Chilean Journal of Agricultural Research Pub Date : 2024-06-01 DOI:10.4067/s0718-58392024000300326

F. Çığ, M. Erman, A. E. Sabagh, M. Ceritoğlu, Sipan Soysal, Özge Uçar, F. Ceritoglu, Rukiye Kiliç, Rojin Özek

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引用次数: 0

Abstract

The objective of this experiment was to determine drought tolerance exhibited by lentil lines developed by the International Center for Agricultural Research in the Dry Areas (ICARDA) in an artificial environment, and to investigate the relationships between traits with various methods. This experiment examined 15 traits of 21 lentil ( Lens culinaris Medik.) accessions grown under artificial drought stress created with polyethylene glycol (PEG)-6000 (0%, 10%, 15% and 20%) concentrations during germination and early seedling stages. Germination characteristics, seedling developmental properties and root system architecture traits were investigated to observed the impacts of drought stress. The originality lies in enabling the identification of drought-tolerant and sensitive genotypes through a brief and practical research method, while shedding light on the key traits by principal component analysis. The first two PCs explained 22.9% and 31.7% (total 54.6%) under optimal conditions while they described 14% and 58.3% (total 72.3%) under PEG-induced drought conditions, respectively. Variation in PC1 was mostly contributed by positive coefficients of germination index, uniformity of germination and germination energy, and negative coefficients of mean germination time. Variation in PC2 was mostly contributed by positive coefficients of seedling vigor index, root fresh weight and root dry weight. ‘Tigris’, G3664 and G3840 exhibited higher performance in terms of germination characteristics, while G3710, G3829 and G3840 produced higher DM accumulation, total biomass and lateral roots. Overall, PC-biplot denoted that selection based on germination index and seedling vigor index at germination and seedling stages would improve drought tolerance. In conclusion, genotypes G3840 and G3664 were identified as drought-tolerant, whereas genotypes G35, G3659, G3759, G3837, and G3844 were classified as drought-sensitive. In addition, G3664, G3840 and G3710 exhibited the highest stress tolerance index (STI) under artificial drought conditions.

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在 PEG 诱导的干旱胁迫下，筛选发芽和早期生长阶段的扁豆基因型

本实验的目的是测定国际干旱地区农业研究中心（ICARDA）开发的小扁豆品系在人工环境中表现出的耐旱性，并通过各种方法研究性状之间的关系。本实验考察了 21 个小扁豆（Lens culinaris Medik.）品系在人工干旱胁迫下生长的 15 个性状，这些胁迫是在发芽和幼苗期使用聚乙二醇（PEG）-6000（0%、10%、15% 和 20%）浓度造成的。通过研究发芽特征、幼苗发育特性和根系结构特征，观察干旱胁迫的影响。其独创性在于通过简明实用的研究方法鉴定了耐旱和敏感基因型，同时通过主成分分析揭示了关键性状。前两个 PC 在最佳条件下分别解释了 22.9% 和 31.7%（总计 54.6%）的性状，而在 PEG 诱导的干旱条件下则分别解释了 14% 和 58.3%（总计 72.3%）的性状。PC1 的变异主要由发芽指数、发芽均匀度和发芽能的正系数以及平均发芽时间的负系数造成。PC2 的变异主要由幼苗活力指数、根鲜重和根干重的正系数造成。'Tigris'、G3664 和 G3840 在发芽特性方面表现较好，而 G3710、G3829 和 G3840 产生的 DM 积累、总生物量和侧根较多。总之，PC-双线图表明，在发芽期和幼苗期根据发芽指数和幼苗活力指数进行选择可提高耐旱性。总之，基因型 G3840 和 G3664 被认定为耐旱，而基因型 G35、G3659、G3759、G3837 和 G3844 被归类为对干旱敏感。此外，在人工干旱条件下，G3664、G3840 和 G3710 表现出最高的抗逆性指数（STI）。

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来源期刊

Chilean Journal of Agricultural Research 农林科学-农艺学

CiteScore

3.00

自引率

11.80%

发文量

审稿时长

6 months

期刊介绍： ChileanJAR publishes original Research Articles, Scientific Notes and Reviews of agriculture, multidisciplinary and agronomy: plant production, plant protection, genetic resources and biotechnology, water management, soil sciences, environment, agricultural economics, and animal production (focused in ruminant feeding). The editorial process is a double-blind peer reviewing, Editorial Office checks format, composition, and completeness, which is a requirement to continue the editorial process. Editorial Committee and Reviewers evaluate relevance and scientific merit of manuscript.