Study of Chlorella vulgaris from Different Growth Phases as Biosensor for Detection of Titanium and Silver Nanoparticles in Water

Q4 Environmental Science

Nature Environment and Pollution Technology Pub Date : 2024-06-01 DOI:10.46488/nept.2024.v23i02.030

Arularasi Thenarasu, M. Chai, Y. Tan, Ling Shing Wong, R. Rajamani, S. Djearamane

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引用次数: 0

Abstract

The increased use of metallic nanoparticles has led to concern for environmental contamination and disruption in water quality. Therefore, effective screening of metallic nanoparticles is important for detecting metallic nanoparticles in aquatic environments. Biosensors offer several advantages, including high sensitivity to pollutants, short response time, energy efficiency, and low waste generation. In this study, a whole-cell biosensor was developed using microalga Chlorella vulgaris as a recognition element, and its fluorescence response was used as a measuring parameter for detecting the presence of titanium dioxide (TiO2) and silver (Ag) nanoparticles in water. The responses of C. vulgaris at the lag, exponential, and stationary phases to different concentrations of TiO2 and Ag nanoparticles were studied. The results showed that in TiO2 and Ag nanoparticles exposures, the highest fluorescence change (50-150%) was observed at the lag phase, whereas the lowest fluorescence change (40-75%) was observed at the stationary phase. A significant fluorescence change was observed in 15 min. The immobilized C. vulgaris under TiO2 and Ag nanoparticles exposures showed 30-180% higher fluorescence change than the negative control, indicating the potential of C. vulgaris as a biosensor for rapid detection of TiO2 and Ag nanoparticles in water. The mathematical modeling of the responses of C. vulgaris to TiO2 and Ag nanoparticles at 15 min of exposure with high R2 indicated that this biosensor is sensitive to the concentration tested (0.010–10.000 mg.L-1). Taken together, these results reveal that, for the first time, it is possible to detect TiO2 and Ag nanoparticles in water within a very short time using a microalgae-based biosensor. Moreover, no genetic engineering requirement makes this biosensor simple, economical, and free from the restriction on genetically modified microorganisms for environmental applications.

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不同生长阶段的小球藻作为生物传感器检测水中钛和银纳米颗粒的研究

金属纳米粒子的使用越来越多，导致了人们对环境污染和水质破坏的担忧。因此，有效筛选金属纳米粒子对于检测水生环境中的金属纳米粒子非常重要。生物传感器具有多种优势，包括对污染物的灵敏度高、响应时间短、能效高和废物产生量少。本研究以微藻类绿球藻（Chlorella vulgaris）为识别元件，开发了一种全细胞生物传感器，并将其荧光响应作为检测水中是否存在二氧化钛（TiO2）和银（Ag）纳米粒子的测量参数。研究了褐藻在滞后期、指数期和静止期对不同浓度的二氧化钛和银纳米粒子的反应。结果表明，在接触二氧化钛和银纳米粒子时，滞后期的荧光变化最大（50-150%），而静止期的荧光变化最小（40-75%）。在 15 分钟内观察到明显的荧光变化。与阴性对照相比，固定化的 C. vulgaris 在 TiO2 和 Ag 纳米粒子暴露下的荧光变化高出 30-180%，这表明 C. vulgaris 具有作为生物传感器快速检测水中 TiO2 和 Ag 纳米粒子的潜力。在 15 分钟的暴露时间内，C. vulgaris 对 TiO2 和 Ag 纳米粒子的反应的数学模型具有很高的 R2，表明该生物传感器对测试浓度（0.010-10.000 mg.L-1）很敏感。综上所述，这些结果首次揭示了利用基于微藻的生物传感器在极短的时间内检测水中 TiO2 和 Ag 纳米粒子的可能性。此外，这种生物传感器不需要基因工程，因此简单、经济，而且不受环境应用中转基因微生物的限制。

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来源期刊

Nature Environment and Pollution Technology Environmental Science-Environmental Science (all)

CiteScore

1.20

自引率

0.00%

发文量

159

审稿时长

36 weeks

期刊介绍： The journal was established initially by the name of Journal of Environment and Pollution in 1994, whose name was later changed to Nature Environment and Pollution Technology in the year 2002. It has now become an open access online journal from the year 2017 with ISSN: 2395-3454 (Online). The journal was established especially to promote the cause for environment and to cater the need for rapid dissemination of the vast scientific and technological data generated in this field. It is a part of many reputed international indexing and abstracting agencies. The Journal has evoked a highly encouraging response among the researchers, scientists and technocrats. It has a reputed International Editorial Board and publishes peer reviewed papers. The Journal has also been approved by UGC (India). The journal publishes both original research and review papers. The ideology and scope of the Journal includes the following. -Monitoring, control and management of air, water, soil and noise pollution -Solid waste management -Industrial hygiene and occupational health -Biomedical aspects of pollution -Toxicological studies -Radioactive pollution and radiation effects -Wastewater treatment and recycling etc. -Environmental modelling -Biodiversity and conservation -Dynamics and behaviour of chemicals in environment -Natural resources, wildlife, forests and wetlands etc. -Environmental laws and legal aspects -Environmental economics -Any other topic related to environment