Enhancing the biodegradation of bis(2-hydroxyethyl) terephthalate by an IsPETasePA and MHETase dual-enzyme system

IF 2.8 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yingtong Gao, Yunxin Zheng, Zixin Qi, Yufan Pan, Yu Zhou, Shengping You, Rongxin Su, Wei Qi, Mengfan Wang
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引用次数: 0

Abstract

BACKGROUND

Environmental and ecological hazards caused by the accumulation of polyethylene terephthalate (PET) are becoming a global concern. The use of enzymes to address the plastic crisis has achieved many successes, but the difficulty in degrading high-crystallinity PET has limited its application. Numerous studies have investigated the degradation of PET with arbitrary crystallinity to bis-2-(2-hydroxyethyl) terephthalate (BHET) using chemical pre-treatment methods such as glycolysis, but few have tested its biocompatibility with enzymatic alliances.

RESULTS

Herein, we report the enzymatic characterization and subsequent engineering of the state-of-the-art IsPETasePA and MHETase (where MHET is mono(2-hydroxyethyl) terephthalate), a dual-enzyme system which can be used for the degradation from BHET to a single-product terephthalate (TPA). Modulators, including surfactants, organic solvents and metal ions, enhanced the enzyme activity of IsPETasePA and MHETase by up to 1.1-fold and 2.3-fold, respectively. 100% TPA yield (BHET of 25 g L−1) was achieved within 5 h. We also analyzed the mechanism of optimal ion modulator modification by dynamics simulation, and it synergistically achieved enhancement of MHET degradation ability by improving stability and binding energy.

CONCLUSION

The introduction of modulators improves the efficiency of the dual-enzyme system in degrading BHET. This work provides a valuable strategy for the complete degradation of BHET to TPA, laying the groundwork for the realization of PET recycling. © 2024 Society of Chemical Industry (SCI).

IsPETasePA 和 MHETase 双酶系统促进对苯二甲酸二(2-羟乙基)酯的生物降解
聚对苯二甲酸乙二醇酯(PET)的累积对环境和生态造成的危害正成为全球关注的问题。使用酶来解决塑料危机已经取得了许多成功,但降解高结晶度 PET 的困难限制了酶的应用。已有大量研究利用化学预处理方法(如糖酵解)将任意结晶度的 PET 降解为对苯二甲酸二-2-(2-羟乙基)酯(BHET),但很少有研究利用酶联盟测试其生物相容性。在此,我们报告了最先进的 IsPETasePA 和 MHETase(其中 MHET 为对苯二甲酸单(2-羟乙基)酯)的酶学特性和后续工程设计,这是一种双酶系统,可用于将 BHET 降解为单产物对苯二甲酸(TPA)。包括表面活性剂、有机溶剂和金属离子在内的调节剂可分别将 IsPETasePA 和 MHETase 的酶活性提高 1.1 倍和 2.3 倍。我们还通过动力学模拟分析了最佳离子调节剂的作用机理,它通过提高稳定性和结合能协同增强了 MHET 的降解能力。这项工作为将 BHET 完全降解为 TPA 提供了一种有价值的策略,为实现 PET 回收奠定了基础。© 2024 化学工业学会(SCI)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.00
自引率
5.90%
发文量
268
审稿时长
1.7 months
期刊介绍: Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.
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