Is genotoxicity a suitable biomarker for monitoring anabolic-androgenic steroids exposure in vivo? A systematic review and meta-analysis.

IF 2.7 4区医学 Q3 TOXICOLOGY

Journal of Applied Toxicology Pub Date : 2024-06-05 DOI:10.1002/jat.4656

Thiago Guedes Pinto, Ingra Tais Malacarne, Wilton Mitsunari Takeshita, Milena de Barros Viana, Ana Claudia Muniz Renno, Daniel Araki Ribeiro

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引用次数: 0

Abstract

Steroids stand for a class of hormones (natural and synthetic) known to be helpful for a number of disorders. Despite the aforementioned beneficial effects of using these hormones, anabolic-androgenic steroids (AAS) are also widely abused in a non-therapeutic manner for muscle-building and strength-increasing properties that may lead to genotoxicity in different tissues. The present study aims to understand whether genotoxicity may be a suitable biomarker for AAS exposure in vivo in both experimental animal and human studies. All studies published in PubMed/Medline, Scopus, and Web of Science electronic databases that presented data on DNA damage caused by AAS were analyzed. A total of 15 articles were included in this study, and after thoroughly reviewing the studies, a total of 8 articles were classified as Strong, 6 were classified as Moderate, and only 1 was classified as Weak, totaling 14 studies being considered either Strong or Moderate. This classification makes it possible to consider the present findings as reliable. The meta-analysis data revealed a statistically significant difference in Wistar rat testis cells with AAS compared to control for tail length and % tail DNA (p < 0.001), so that the selected articles were considered homogeneous and the I² of 0% indicated low heterogeneity. In summary, genotoxicity can be considered a suitable biomarker for monitoring AAS exposure as a result of DNA breakage and oxidative DNA damage.

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基因毒性是监测体内合成代谢雄性类固醇暴露的合适生物标志物吗？系统回顾和荟萃分析。

类固醇是一类激素（天然的和合成的），已知对多种疾病有帮助。尽管使用这些激素有上述益处，但合成代谢雄性类固醇（AAS）也因其增肌和增强力量的特性而被广泛滥用，并可能导致不同组织的基因毒性。本研究旨在了解在实验动物和人体研究中，基因毒性是否可作为体内暴露于合成类固醇的合适生物标志物。本研究分析了在PubMed/Medline、Scopus和Web of Science电子数据库中发表的所有关于AAS导致DNA损伤的研究。本研究共收录了 15 篇文章，在对这些研究进行全面审查后，共有 8 篇文章被归类为 "强"，6 篇文章被归类为 "中"，只有 1 篇文章被归类为 "弱"，共有 14 项研究被认为是 "强 "或 "中"。这样的分类使我们可以认为目前的研究结果是可靠的。荟萃分析数据显示，与对照组相比，使用 AAS 的 Wistar 大鼠睾丸细胞在尾长和尾 DNA 百分比方面存在显著的统计学差异（p 2 为 0%，表明异质性较低）。总之，由于 DNA 断裂和氧化 DNA 损伤，基因毒性可被视为监测 AAS 暴露的合适生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Toxicology 医学-毒理学

CiteScore

7.00

自引率

6.10%

发文量

145

审稿时长

1 months

期刊介绍： Journal of Applied Toxicology publishes peer-reviewed original reviews and hypothesis-driven research articles on mechanistic, fundamental and applied research relating to the toxicity of drugs and chemicals at the molecular, cellular, tissue, target organ and whole body level in vivo (by all relevant routes of exposure) and in vitro / ex vivo. All aspects of toxicology are covered (including but not limited to nanotoxicology, genomics and proteomics, teratogenesis, carcinogenesis, mutagenesis, reproductive and endocrine toxicology, toxicopathology, target organ toxicity, systems toxicity (eg immunotoxicity), neurobehavioral toxicology, mechanistic studies, biochemical and molecular toxicology, novel biomarkers, pharmacokinetics/PBPK, risk assessment and environmental health studies) and emphasis is given to papers of clear application to human health, and/or advance mechanistic understanding and/or provide significant contributions and impact to their field.