Methylation of Long Noncoding RNA Genes SNHG6, SNHG12, and TINCR in Ovarian Cancer

IF 1.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biology Pub Date : 2024-06-05 DOI:10.1134/s0026893324700067

S. S. Lukina, A. M. Burdennyy, E. A. Filippova, L. A. Uroshlev, I. V. Pronina, N. A. Ivanova, M. V. Fridman, K. I. Zhordania, T. P. Kazubskaya, N. E. Kushlinskii, V. I. Loginov, E. A. Braga

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Abstract

Ovarian cancer (OC) develops asymptomatically and escapes diagnosis until advanced stages, the feature contributing to a higher mortality rate. New prospects of OC diagnosis and treatment have been opened in studies of the gene regulation mechanisms that involve long noncoding RNAs (lncRNAs) and identification of the lncRNA genes that are inhibited via methylation of the promoter region. A set of 122 samples of primary OC tumors was examined by methylation specific real-time PCR to assess the methylation level of the lncRNA genes PLUT, SNHG1, SNHG6, SNHG12, and TINCR. A significant increase in their methylation levels was observed in OC (p < 0.001 by the nonparametric Mann–Whitney test). The methylation levels of SNHG6, SNHG12, and TINCR were found to correlate significantly (p < 0.05) with the stage of the tumor process, the histological grade, and metastasis. Downregulation of SNHG6, SNHG12, and TINCR was detected by real-time RT–qPCR, and a significant correlation between methylation and expression was demonstrated for SNHG6 and TINCR (r_s ≤ –0.5, p < 0.001). The respective lncRNA genes were assumed to provide potential epigenetic markers of OC.

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卵巢癌中长非编码 RNA 基因 SNHG6、SNHG12 和 TINCR 的甲基化情况

摘要卵巢癌（Ovarian cancer，OC）无症状发展，直到晚期才被诊断出来，这一特点导致了较高的死亡率。对长非编码 RNA（lncRNA）基因调控机制的研究以及通过启动子区域甲基化抑制的 lncRNA 基因的鉴定为卵巢癌的诊断和治疗开辟了新的前景。研究人员利用甲基化特异性实时荧光定量PCR技术检测了一组122个原发性OC肿瘤样本，以评估lncRNA基因PLUT、SNHG1、SNHG6、SNHG12和TINCR的甲基化水平。在 OC 中观察到这些基因的甲基化水平明显增加（通过非参数 Mann-Whitney 检验，p < 0.001）。研究发现，SNHG6、SNHG12 和 TINCR 的甲基化水平与肿瘤病程分期、组织学分级和转移有明显相关性（p < 0.05）。通过实时 RT-qPCR 检测到了 SNHG6、SNHG12 和 TINCR 的下调，SNHG6 和 TINCR 的甲基化与表达之间存在显著相关性（rs ≤ -0.5，p <0.001）。推测相应的 lncRNA 基因可能是 OC 的表观遗传标记。

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来源期刊

Molecular Biology 生物-生化与分子生物学

CiteScore

1.30

自引率

8.30%

发文量

审稿时长

3 months

期刊介绍： Molecular Biology is an international peer reviewed journal that covers a wide scope of problems in molecular, cell and computational biology including genomics, proteomics, bioinformatics, molecular virology and immunology, molecular development biology, molecular evolution and related areals. Molecular Biology publishes reviews, experimental and theoretical works. Every year, the journal publishes special issues devoted to most rapidly developing branches of physical-chemical biology and to the most outstanding scientists.