Investigating the Influence of Temperature and Supplementation Timing on Antifungal Efficacy in Storage Medium for Corneal Transplantation.

IF 2.6 3区医学 Q2 OPHTHALMOLOGY

Ophthalmology and Therapy Pub Date : 2024-08-01 Epub Date: 2024-06-03 DOI:10.1007/s40123-024-00969-0

Hsin-Yu Liu, Pao-Yu Chen, Hsiao-Sang Chu, Ya-Ting Chiu, Yee-Chun Chen, Fung-Rong Hu

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Abstract

Introduction: Although antifungal supplementation reduces the fungal load in the corneal storage medium, consensus is lacking on the influence of dosing and temperature. The study aims to evaluate the impact of eye bank warming protocol and timing of antifungal supplements on efficacy in Optisol-GS and tissue.

Methods: Corneoscleral rims contaminated with Candida albicans (C. albicans) were incubated in Optisol-GS, either without antifungal agents or with the addition of amphotericin B or voriconazole at various concentrations (2 ×, 5 ×, 10 ×, and 20 × MIC), at different time points, and under various preservation temperatures (2-8 °C versus 2 h-room temperature exposure). Antifungal efficacy was evaluated by counting viable yeast colonies cultured from Optisol-GS samples. Tissue sterility was determined through direct tissue culture and histological examination of the contaminated rims after a 14-day incubation period.

Results: Room temperature exposure did not increase colony growth at the same multiple MIC of antifungal agents. Although antifungal addition reduced C. albicans growth in a concentration-dependent manner, yeast growth was still observed in all Optisol-GS samples with a single supplementation after a 14-day incubation. Only groups with additional antifungal supplementation on either day 2 or day 6 showed a 99% or greater reduction of C. albicans growth in Optisol-GS samples and yielded negative results in direct tissue culture.

Conclusions: The eye bank warming protocol did not compromise antifungal efficacy. To sustain the required concentration and effectively reduce C. albicans growth in Optisol-GS and contaminated tissue, additional antifungal supplementation on either day 2 or day 6 was necessary during a 2-week preservation period.

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研究温度和补充时间对角膜移植储存介质抗真菌功效的影响

简介：尽管抗真菌补充剂可减少角膜储存介质中的真菌负荷，但对剂量和温度的影响尚缺乏共识。本研究旨在评估眼库加温方案和补充抗真菌剂的时间对 Optisol-GS 和组织疗效的影响：方法：将被白色念珠菌（C. albicans）污染的角膜巩膜边缘放入 Optisol-GS 中培养，在不同的时间点和不同的保存温度（2-8 °C 与室温暴露 2 小时）下，不添加抗真菌剂或添加不同浓度（2 ×、5 ×、10 × 和 20 × MIC）的两性霉素 B 或伏立康唑。抗真菌效果通过计数从 Optisol-GS 样品中培养出的有活力酵母菌菌落进行评估。组织无菌性是通过直接组织培养和 14 天培养期后对受污染的边缘进行组织学检查来确定的：结果：在相同的多重 MIC 抗真菌剂条件下，室温暴露不会增加菌落的生长。虽然抗真菌剂的添加以浓度依赖的方式减少了白僵菌的生长，但在培养 14 天后，所有添加了单一抗真菌剂的 Optisol-GS 样品中仍能观察到酵母菌的生长。只有在第 2 天或第 6 天补充抗真菌剂的组别才显示 Optisol-GS 样品中的白僵菌生长减少了 99% 或更多，并在直接组织培养中产生了阴性结果：眼库加温方案不会影响抗真菌效果。要维持所需的浓度并有效减少白僵菌在 Optisol-GS 和受污染组织中的生长，必须在 2 周的保存期内于第 2 天或第 6 天补充抗真菌剂。

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来源期刊

Ophthalmology and Therapy OPHTHALMOLOGY-

CiteScore

4.20

自引率

3.00%

发文量

157

审稿时长

6 weeks

期刊介绍： Aims and Scope Ophthalmology and Therapy is an international, open access, peer-reviewed (single-blind), and rapid publication journal. The scope of the journal is broad and will consider all scientifically sound research from preclinical, clinical (all phases), observational, real-world, and health outcomes research around the use of ophthalmological therapies, devices, and surgical techniques. The journal is of interest to a broad audience of pharmaceutical and healthcare professionals and publishes original research, reviews, case reports/series, trial protocols and short communications such as commentaries and editorials. Ophthalmology and Therapy will consider all scientifically sound research be it positive, confirmatory or negative data. Submissions are welcomed whether they relate to an international and/or a country-specific audience, something that is crucially important when researchers are trying to target more specific patient populations. This inclusive approach allows the journal to assist in the dissemination of quality research, which may be considered of insufficient interest by other journals. Rapid Publication The journal’s publication timelines aim for a rapid peer review of 2 weeks. If an article is accepted it will be published 3–4 weeks from acceptance. The rapid timelines are achieved through the combination of a dedicated in-house editorial team, who manage article workflow, and an extensive Editorial and Advisory Board who assist with peer review. This allows the journal to support the rapid dissemination of research, whilst still providing robust peer review. Combined with the journal’s open access model this allows for the rapid, efficient communication of the latest research and reviews, fostering the advancement of ophthalmic therapies. Open Access All articles published by Ophthalmology and Therapy are open access. 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