{"title":"Extraction of genomic DNA for sequencing from snail <i>Helix lucorum</i>.","authors":"Dmitry Panteleev, Anastasia Sadova, Galina Pavlova","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Genomic studies make it possible to breakthrough in many fields such as biochemistry, physiology, phylogenetics, etc., though\nthey are unworkable without sequences of genomic DNA of an organism. The terrestrial mollusks’ genomes would benefit gastropod\nbiology investigations, that are unavailable so far due to problems in DNA integrity and quality after the isolation procedures. Here we\ndescribe a fast and handy protocol for genomic DNA extraction from the tissues of <i>Helix lucorum</i>, which allows to yield high-quality\nsamples applicable for downstream analysis such as high-throughput DNA sequencing. Troubleshooting revealed the nuclease activity of\nsnail tissue lysate, which may be avoided by heating the lysate and decreasing the incubation time.</p>","PeriodicalId":15907,"journal":{"name":"Journal of Genetics","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Genetics","FirstCategoryId":"99","ListUrlMain":"","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"EDUCATION & EDUCATIONAL RESEARCH","Score":null,"Total":0}
引用次数: 0
Abstract
Genomic studies make it possible to breakthrough in many fields such as biochemistry, physiology, phylogenetics, etc., though
they are unworkable without sequences of genomic DNA of an organism. The terrestrial mollusks’ genomes would benefit gastropod
biology investigations, that are unavailable so far due to problems in DNA integrity and quality after the isolation procedures. Here we
describe a fast and handy protocol for genomic DNA extraction from the tissues of Helix lucorum, which allows to yield high-quality
samples applicable for downstream analysis such as high-throughput DNA sequencing. Troubleshooting revealed the nuclease activity of
snail tissue lysate, which may be avoided by heating the lysate and decreasing the incubation time.
期刊介绍：
The journal retains its traditional interest in evolutionary research that is of relevance to geneticists, even if this is not explicitly genetical in nature. The journal covers all areas of genetics and evolution,including molecular genetics and molecular evolution.It publishes papers and review articles on current topics, commentaries and essayson ideas and trends in genetics and evolutionary biology, historical developments, debates and book reviews. From 2010 onwards, the journal has published a special category of papers termed ‘Online Resources’. These are brief reports on the development and the routine use of molecular markers for assessing genetic variability within and among species. Also published are reports outlining pedagogical approaches in genetics teaching.
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