The Effect of Bacillus Cereus on the Ion Homeostasis, Growth Parameters, and the Expression of Some Genes of Artemisinin Biosynthesis Pathway in Artemisia Absinthium Under Salinity Stress.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Iranian Journal of Biotechnology Pub Date : 2024-01-01 DOI:10.30498/ijb.2024.394178.3687

Sara Salimian Rizi, Zahra Rezayatmand, Monireh Ranjbar, Nasrin Yazdanpanahi, Zarrin Dokht Emami-Karvani

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Abstract

Background: Soil salinity is a major problem in the world that affects the growth and yield of plants. Application of new and up-to-date techniques can help plants in dealing with salinity stress. One of the approaches for reducing environmental stress is the use of rhizosphere bacteria.

Objective: The aim of present study was to investigate the effect of the inoculation of Bacillus cereus on physiological and biochemical indicators and the expression of some key genes involved in the Artemisinin biosynthesis pathway in Artemisia absinthium under salinity stress.

Materials and methods: The study was conducted using three different salinity levels (0, 75, 150 mM/NaCl) and two different bacterial treatments (i. e, without bacterial inoculation and co-inoculation with B. cereus isolates). The data from the experiments were analyzed using factorial analysis, and the resulting interaction effects were subsequently examined and discussed.

Results: The results showed that with increasing salinity, root and stem length, root and stem weight, root and stem dry weight, and potassium content were decreased, although the content of sodium was increased. Rhizosphere bacteria increased the contents of Artemisinin, potassium, calcium, magnesium, and iron and the expression of Amorpha-4,11-diene synthase and Cytochrome P450 monooxygenase1 genes as well as the growth indicators; although decreased the sodium content. The highest ADS expression was related to co-inoculation with B. cereus isolates E and B in 150 mM salinity. The highest CYP71AV1 expression was related to co-inoculation with B. cereus isolates E and B in 150 mM salinity.

Conclusion: These findings showed that the increase in growth indices under salinity stress was probably due to the improvement of nutrient absorption conditions as a result of ion homeostasis, sodium ion reduction and Artemisinin production conditions by rhizosphere B. cereus isolates E and B.

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盐度胁迫下枯草芽孢杆菌对青蒿体内离子平衡、生长参数及青蒿素合成途径部分基因表达的影响

背景：土壤盐碱化是影响植物生长和产量的一个主要问题。应用最新的技术可以帮助植物应对盐分胁迫。减少环境压力的方法之一是利用根瘤菌：本研究旨在探讨接种蜡样芽孢杆菌对盐胁迫下苦艾蒿生理生化指标的影响以及参与青蒿素生物合成途径的一些关键基因的表达：研究采用了三种不同的盐度水平（0、75、150 mM/NaCl）和两种不同的细菌处理方法（即不接种细菌和与 B. cereus 分离物联合接种）。采用因子分析法对实验数据进行了分析，并对由此产生的交互效应进行了研究和讨论：结果表明，随着盐度的增加，根和茎的长度、根和茎的重量、根和茎的干重以及钾的含量都有所减少，但钠的含量有所增加。根瘤菌提高了青蒿素、钾、钙、镁和铁的含量，增加了Amorpha-4,11-二烯合成酶和细胞色素P450单氧化酶1基因的表达量以及生长指标，但降低了钠的含量。在 150 mM 的盐度条件下，ADS 的最高表达量与 B. cereus 分离物 E 和 B 共同接种有关。CYP71AV1 的最高表达与在 150 mM 盐度下与蜡状芽孢杆菌分离物 E 和 B 共同接种有关：这些研究结果表明，盐胁迫下生长指数的提高可能是由于根瘤芽孢杆菌分离物 E 和 B 改善了离子平衡、钠离子还原和青蒿素生产条件，从而改善了营养吸收条件。

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来源期刊

Iranian Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

7.70%

发文量

期刊介绍： Iranian Journal of Biotechnology (IJB) is published quarterly by the National Institute of Genetic Engineering and Biotechnology. IJB publishes original scientific research papers in the broad area of Biotechnology such as, Agriculture, Animal and Marine Sciences, Basic Sciences, Bioinformatics, Biosafety and Bioethics, Environment, Industry and Mining and Medical Sciences.