Stopped-flow measurement of CO2 hydration activity by catalytic amyloids.

4区 生物学 Q3 Biochemistry, Genetics and Molecular Biology
Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-03-21 DOI:10.1016/bs.mie.2024.01.016
Liam R Marshall, Olga V Makhlynets
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引用次数: 0

Abstract

With the ever-increasing rates of catalysis shown by catalytic amyloids, the use of faster characterization techniques is required for proper kinetic studies. The same is true for inherently fast chemical reactions. Carbon dioxide hydration is of significant interest to the field of enzyme design, given both carbonic anhydrases' status as a "perfect enzyme" and the central role carbonic anhydrase plays in the respiration and existence of all carbon-based life. Carbon dioxide is an underexplored hydrolysis substrate within the literature, and a lack of a direct spectroscopic marker for reaction monitoring can make studies more complex and require specialist equipment. Within this article we present a method for measuring the carbon dioxide hydration activity of amyloid fibrils.

通过催化淀粉样蛋白对二氧化碳水合活性进行停流测量。
随着催化淀粉的催化速率不断提高,需要使用更快的表征技术来进行适当的动力学研究。固有的快速化学反应也是如此。鉴于碳酸酐酶作为 "完美酶 "的地位,以及碳酸酐酶在所有碳基生命的呼吸和存在中发挥的核心作用,二氧化碳水合作用对酶设计领域具有重大意义。在文献中,二氧化碳是一种未被充分开发的水解底物,由于缺乏用于监测反应的直接光谱标记,研究变得更加复杂,并且需要专业设备。本文介绍了一种测量淀粉样蛋白纤维的二氧化碳水合活性的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Methods in enzymology
Methods in enzymology 生物-生化研究方法
CiteScore
2.90
自引率
0.00%
发文量
308
审稿时长
3-6 weeks
期刊介绍: The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.
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