The profile of cytokines against bacterial infection in dental pulp

IF 3.2 4区 医学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Zhongcheng Bai, Jun Liu, Hehuizi Bai
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引用次数: 0

Abstract

Background

Dental pulp in a confined environment, with little connection to the outside and only a small distribution of immune cells, provides a good research model for investigating how cells respond to bacterial infections through cytokines.

Methods

The data of single-cell transcriptome sequencing of healthy and inflamed pulp tissue were downloaded from the GEO dataset. The expression character of 79 cytokines was analyzed based on the expression matrix.

Results

The cytokine secretion profiles of the two populations of pulp cells in healthy dental pulp were associated with vascularization and nervous system development, as well as immune cell regulation. For the three populations of pulp stem cells with stem cell activity in the dental pulp, the secretion of cytokines related to nervous system development, regulation of endothelial cell proliferation and migration, and regulation of immune cell function comprised the characteristics that we observed. The cytokines secreted by T cells and macrophages were more of an immune reserve against pathogenic microorganisms. In the inflammatory state, the spectrum of cytokines secreted by various types of cells in the dental pulp tended to be identical, such that it mainly resisted pathogenic microorganisms.

Conclusions

The cytokine secretion profiles of various cell types in healthy and inflamed dental pulp at the single-cell level are summarized.

Abstract Image

细胞因子对牙髓细菌感染的影响。
背景:牙髓处于一个封闭的环境中,与外界几乎没有联系,只有少量的免疫细胞分布,这为研究细胞如何通过细胞因子对细菌感染做出反应提供了一个很好的研究模型:方法:从 GEO 数据集中下载了健康牙髓组织和发炎牙髓组织的单细胞转录组测序数据。方法:从 GEO 数据集中下载了健康和发炎牙髓组织的单细胞转录组测序数据,并根据表达矩阵分析了 79 种细胞因子的表达特征:结果:健康牙髓中两组牙髓细胞的细胞因子分泌特征与血管形成、神经系统发育以及免疫细胞调节有关。对于牙髓中具有干细胞活性的三组牙髓干细胞,我们观察到的细胞因子分泌特征包括与神经系统发育有关的细胞因子、调节内皮细胞增殖和迁移的细胞因子以及调节免疫细胞功能的细胞因子。T 细胞和巨噬细胞分泌的细胞因子更多的是针对病原微生物的免疫储备。在炎症状态下,牙髓中各类细胞分泌的细胞因子谱趋于一致,因此主要是抵抗病原微生物:总结了健康牙髓和炎症牙髓中各类细胞在单细胞水平上的细胞因子分泌谱。
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来源期刊
Journal of Gene Medicine
Journal of Gene Medicine 医学-生物工程与应用微生物
CiteScore
6.40
自引率
0.00%
发文量
80
审稿时长
6-12 weeks
期刊介绍: The aims and scope of The Journal of Gene Medicine include cutting-edge science of gene transfer and its applications in gene and cell therapy, genome editing with precision nucleases, epigenetic modifications of host genome by small molecules, siRNA, microRNA and other noncoding RNAs as therapeutic gene-modulating agents or targets, biomarkers for precision medicine, and gene-based prognostic/diagnostic studies. Key areas of interest are the design of novel synthetic and viral vectors, novel therapeutic nucleic acids such as mRNA, modified microRNAs and siRNAs, antagomirs, aptamers, antisense and exon-skipping agents, refined genome editing tools using nucleic acid /protein combinations, physically or biologically targeted delivery and gene modulation, ex vivo or in vivo pharmacological studies including animal models, and human clinical trials. Papers presenting research into the mechanisms underlying transfer and action of gene medicines, the application of the new technologies for stem cell modification or nucleic acid based vaccines, the identification of new genetic or epigenetic variations as biomarkers to direct precision medicine, and the preclinical/clinical development of gene/expression signatures indicative of diagnosis or predictive of prognosis are also encouraged.
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