Kyungho Won, Eu Ddeum Choi, Keumsun Kim, Il Sheob Shin, Seongsig Hong, Cécile Segonzac, Kee Hoon Sohn, Cecilia H. Deng, Lester Brewer, David Chagné, Vincent G. M. Bus
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引用次数: 0
Abstract
Pear scab is a major disease of pear worldwide and is caused by two distinct species that are aligned with different commercial Pyrus species: Venturia pirina with European pear (P. communis) and V. nashicola with Asian pear species P. pyrifolia, P. ussuriensis and P. X bretschneideri. As these host-pathogen systems are mutually exclusive, interspecific pear breeding provides an avenue for breeding new scab-resistant cultivars. Here we describe the genetic mapping of resistance to V. nashicola in a pear progeny between interspecific pear selection P019R045T042 and ‘Shinko’ (P. pyrifolia) consisting of 274 seedlings, which was phenotyped twice with V. nashicola inoculum prepared from scab-infected leaves collected from trees of susceptible ‘Niitaka’ (P. pyrifolia). A set of 613 polymorphic DNA markers were selected from the apple and pear Illumina Infinium® II 9K single nucleotide polymorphism array and genotyping-by-sequencing for the creation of the parental genetic maps with JoinMap v4.1. Using the Interval Mapping module in MapQTL v6.0 software, two significant quantitative trait loci were detected in P019R045T042: one on linkage group (LG) 7 and another on LG10, which we name Rvn5 and Rvn6, respectively. Both Rvn5 and Rvn6 displayed weak additive effects for pear scab (V. nashicola) resistance when both loci worked together in this family. They will contribute to more strong resistance based on gene pyramiding through marker-assisted selections for the introduction of non-host resistance into both Asian and European pears through interspecific hybridization.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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