{"title":"D-Ribose-L-Cysteine and L-Dopa Co-Supplementation Attenuates Paraquat-Induced Oxidative Damage and Locomotion Deficit in Drosophila melanogaster","authors":"Adeola Oluwatosin Adedara, Grace Temitope Akingbade","doi":"10.1134/s1819712424010021","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>D-ribose L-cysteine is a glutathione precursor that acts as an antioxidant. In the present study, we investigated the effect of D-ribose L-cysteine and L-dopa combination against paraquat toxicity in <i>Drosophila melanogaster</i>. Adult wild-type flies were exposed to Paraquat (500 μM), RLC (250 μM), and/or L-dopa (0.1 mM) in their diet for 7 days. The survival rate, locomotion activities, and markers of antioxidants (Catalase, glutathione-<i>S</i>-transferase, superoxide dismutase, thioredoxin reductase, total and non-protein thiol), oxidative stress (hydrogen peroxide, protein carbonyl, malondialdehyde, nitrite/nitrates), and dehydrogenase activity were measured in the flies. The results showed a non-toxic effect of the combination of RLC and L-dopa. In addition, elevated oxidative stress markers due to paraquat treatments were significantly reduced after treatments with RLC and L-dopa. Combination treatment also significantly restored altered antioxidant status, improved survival rate, and impaired locomotion induced by paraquat. This study suggests that RLC could serve as adjunct therapy with L-dopa in managing Parkinson’s disease and toxicity associated with paraquat and related compounds</p>","PeriodicalId":19119,"journal":{"name":"Neurochemical Journal","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurochemical Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1134/s1819712424010021","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
D-ribose L-cysteine is a glutathione precursor that acts as an antioxidant. In the present study, we investigated the effect of D-ribose L-cysteine and L-dopa combination against paraquat toxicity in Drosophila melanogaster. Adult wild-type flies were exposed to Paraquat (500 μM), RLC (250 μM), and/or L-dopa (0.1 mM) in their diet for 7 days. The survival rate, locomotion activities, and markers of antioxidants (Catalase, glutathione-S-transferase, superoxide dismutase, thioredoxin reductase, total and non-protein thiol), oxidative stress (hydrogen peroxide, protein carbonyl, malondialdehyde, nitrite/nitrates), and dehydrogenase activity were measured in the flies. The results showed a non-toxic effect of the combination of RLC and L-dopa. In addition, elevated oxidative stress markers due to paraquat treatments were significantly reduced after treatments with RLC and L-dopa. Combination treatment also significantly restored altered antioxidant status, improved survival rate, and impaired locomotion induced by paraquat. This study suggests that RLC could serve as adjunct therapy with L-dopa in managing Parkinson’s disease and toxicity associated with paraquat and related compounds
期刊介绍：
Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components.
The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.
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