{"title":"Isolation of Phytohormone trans-Zeatin: Potential Oxidant Scavenger and Anti-Aging Compound","authors":"Adarsh Kesharwani, Siva Prasad Panda","doi":"10.1134/s1819712424010112","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>In all parts of the world, dementia rates are rising rapidly, particularly in Western nations, where it accounts for roughly 60% of cases and is linked to population aging. According to the free radical hypothesis of aging, age-related cellular and tissue damages are brought on by free radicals. <i>Cocos nucifera</i> (L<i>.</i>) has been traditionally utilized in various health conditions, including digestive disorders, antifungal, wound healing, immune support, and cardiovascular health. Objective of the research is to isolate, quantify, and characterize <i>trans-</i>Zeatin (tZn) from hydroalcoholic extract of <i>Cocos nucifera</i> (HECN) and to evaluate its anti-aging and antioxidant potential by studying the Keap-1/HO-1 signaling pathway through in-silico docking analysis. UV, IR, HPLC, and NMR techniques were used for isolation and quantification of tZn. The in vitro anti-oxidant potential of tZn was examined, by using the DPPH, OH, and NO radical scavenging assay. Anti-aging potential of tZn was evaluated by docking analysis against selected proteins (Keap-1 and HO-1) using AutoDock Vina software. From the result it was found that tZn has strong antioxidant potential in a concentration dependent manner with IC<sub>50</sub> values of 13.86 ± 2.8, 15.49 ± 3.2, and 16.96 ± 1.5 µg/mL comparable with reference standard ascorbic acid. The molecular docking analysis showed the significant binding potential of the tZn with the active sites of Keap-1 and HO-1, which are crucially involved in the pathophysiology of aging. Based on the conducted studiеs, it is concluded that the tZn from HECN could be measured as a potential anti-oxidant and anti-aging compound.</p>","PeriodicalId":19119,"journal":{"name":"Neurochemical Journal","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurochemical Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1134/s1819712424010112","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
In all parts of the world, dementia rates are rising rapidly, particularly in Western nations, where it accounts for roughly 60% of cases and is linked to population aging. According to the free radical hypothesis of aging, age-related cellular and tissue damages are brought on by free radicals. Cocos nucifera (L.) has been traditionally utilized in various health conditions, including digestive disorders, antifungal, wound healing, immune support, and cardiovascular health. Objective of the research is to isolate, quantify, and characterize trans-Zeatin (tZn) from hydroalcoholic extract of Cocos nucifera (HECN) and to evaluate its anti-aging and antioxidant potential by studying the Keap-1/HO-1 signaling pathway through in-silico docking analysis. UV, IR, HPLC, and NMR techniques were used for isolation and quantification of tZn. The in vitro anti-oxidant potential of tZn was examined, by using the DPPH, OH, and NO radical scavenging assay. Anti-aging potential of tZn was evaluated by docking analysis against selected proteins (Keap-1 and HO-1) using AutoDock Vina software. From the result it was found that tZn has strong antioxidant potential in a concentration dependent manner with IC50 values of 13.86 ± 2.8, 15.49 ± 3.2, and 16.96 ± 1.5 µg/mL comparable with reference standard ascorbic acid. The molecular docking analysis showed the significant binding potential of the tZn with the active sites of Keap-1 and HO-1, which are crucially involved in the pathophysiology of aging. Based on the conducted studiеs, it is concluded that the tZn from HECN could be measured as a potential anti-oxidant and anti-aging compound.
期刊介绍：
Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components.
The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.
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