TRACE ELEMENTS AND BIOLOGICALLY ACTIVE COMPOUNDS OF PULMONARIA MOLLIS AND PULMONARIA OBSCURA

Abstract

The genus Pulmonaria plants are ephemeroids having characteristic distile reproduction system and so should be distinguished – generative shoot with flowers and vegetative rosette leaves as organs these plants. Phytomedicines made from them have different pharmacological action. The aim this work was study of biologically active compounds and trace elements in both kinds of aboveground organs of the Pulmonaria obscura Dumort and Pulmonaria mollis Wulf. Ex.Horn. The content of trace elements was determined by inductively coupled plasma mass spectroscopy. It was determined the content of biogenic for plants trace elements, as well as elements necessary for the process of hematopoiesis in the human body – B, K, P, V, Ca, Co, Cu, Fe, Mg, Mn, Mo, Na, Si, Zn Cr , Ni, Se. The study of the composition of phenolic compounds was carried out on a high performance liquid chromatograph. Polysaccharides, protein and tannins were determined gravimetrically. The presence of alkaloids was checked by reaction with group-wide precipitating reagents. Besides, content of flavonoids was determined spectrophotometrically. It was established: – generative shoots and rosette leaves form separate clusters according to microelements of the hematopoietic complex that correlating with their pharmocological activity; – polysaccharide-protein complex containing up to 20% protein was found and this is impotant for phamacologocal action; – marker compounds for generative shoots are caffeic acid, hyperoside and delphinidin, while rosette leaves are characterized by the presence of vicenin and luteolin-7-O-glycoside; – alkaloids wasn`t find and this fact makes studied plants promising for use in scientific medicine.