ACTION OF EXTRACTS FROM ARTEMİSİA L. ON THE PLASMATIC MEMBRANE UNDER STRESS CONDITIONS

G. Babayeva, N. Musayev
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Abstract

Plant bioactive compounds are supposed to be the modifiers of structural-functional properties of cell plasma membrane under HM contamination. To test a protective effect of extracts from Artemisia L. species, possessing high adaptive potential to different soil contaminations, on electrical properties of algae cells under HM treatments was the aim of the present work. Extracts from Artemisia plants were isolated from above-ground parts of A.scoparia and A.szovitsiana by acetone hydrolyzation. Microelectrode techniques were used to study the regularities of change of membrane potential and membrane resistance of Chara gymnophylla and Nitella flexilis plasmalemma under influence of Cd, Cu, Zn, Pb and the extracts from Artemisia species. Statistically reliable hyperpolarizing effect of A.szovitsiana and A.scoparia extracts in relation to Zn and Pb was revealed, respectively. Elimination by extracts a decrease of membrane conductivity caused by Cd and Zn and also an increase of conductivity by Cu was determined. Though a high HM accumulation capacity of Artemisia L. species is noticed to not connect with protective effect of their extracts on membrane conductivity, they can be a potential source for the searching new chemical compounds which are capable to induce the plant tolerance to stress impacts.
在胁迫条件下,蒿草提取物对质膜的作用
植物生物活性化合物被认为是氢卤素污染下细胞质膜结构功能特性的调节剂。蒿属植物对不同的土壤污染具有很强的适应能力,本研究旨在测试蒿属植物萃取物对 HM 处理下藻类细胞电特性的保护作用。通过丙酮水解法,从蒿属植物的地上部分(A.scoparia 和 A.szovitsiana)中分离出了蒿属植物提取物。使用微电极技术研究了在镉、铜、锌、铅和青蒿提取物的影响下,Chara gymnophylla 和 Nitella flexilis plasmalemma 的膜电位和膜电阻变化的规律性。据统计,A.szovitsiana 和 A.scoparia 提取物分别对锌和铅具有可靠的超极化作用。经测定,萃取物消除了由镉和锌引起的膜传导性下降以及由铜引起的传导性上升。虽然注意到蒿属植物具有较高的 HM 积累能力,但这与它们的萃取物对膜传导性的保护作用无关,它们可以成为寻找新的化学物质的潜在来源,这些化学物质能够提高植物对胁迫影响的耐受性。
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