Frequency of Herpes Simplex Virus Types 1 and 2 and Cytomegalovirus in Perilymph and Peripheral Blood Samples of Cochlear-Implanted Children Using the Polymerase Chain Reaction Method

IF 0.4 Q4 AUDIOLOGY & SPEECH-LANGUAGE PATHOLOGY

Auditory and Vestibular Research Pub Date : 2024-05-21 DOI:10.18502/avr.v33i3.15500

Farhad Farahani, Majid Vafaei Rad, H. Naderifar, Elnaz Shariatpanahi, R. Najafi-Vosough, F. Jalilian

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Abstract

Background and Aim: Inner ear infection with some viruses may be one of the possible causes of Sensorineural Hearing Loss (SNHL). This study aims to determine the frequency of Herpes Simplex Virus 1 and 2 (HSV-1, HSV-2) and Cytomegalovirus (CMV) in perilymph and peripheral blood samples of cochlear-implanted children. Methods: In this cross-sectional study, 30 children with severe-to-profound SNHL (aged 1.1–5 years) underwent cochlear implantation surgery. During surgery, their perilymph and peripheral blood samples were collected. The samples were analyzed separately for the presence of herpes HSV-1, HSV-2, and CMV by real-time Polymerase Chain Reaction (PCR) method. The load of IgG and IgM antibodies against these viruses was determined using the Enzyme-Linked Immunosorbent Assay (ELISA) method. Results: The frequency of CMV in perilymph samples was 16.7% (5 patients) and in peripheral blood samples was 3.3% (1 patient). The IgG antibody against CMV and HSV- 1 was positive in 80% and 46.7% of the patients, respectively. The IgM antibody against CMV was positive in 10%. The mean IgM serum antibody load against HSV-1, HSV-2, and CMV was 2.70, 1.70, and 5.47, respectively, and the mean IgG antibody load against these viruses was 56.07, 2.50, and 23.67, respectively. Conclusion: The IgG test is positive in cochlear-implanted children with CMV in their perilymph samples, and the CMV genome is not present in their peripheral blood. This may indicate the previous presence of this virus in the ear and its role in hearing loss. Keywords: Sensorineural hearing loss; perilymph fluid; cytomegalovirus; herpes simplex

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使用聚合酶链反应法检测人工耳蜗植入儿童耳道和外周血样本中 1 型和 2 型单纯疱疹病毒以及巨细胞病毒的频率

背景和目的：内耳感染某些病毒可能是导致感音神经性听力损失（SNHL）的原因之一。本研究旨在确定单纯疱疹病毒 1 和 2（HSV-1、HSV-2）以及巨细胞病毒（CMV）在人工耳蜗植入儿童的耳周液和外周血样本中的感染频率。研究方法在这项横断面研究中，30 名患有重度至疑似 SNHL 的儿童（1.1-5 岁）接受了人工耳蜗植入手术。手术期间，采集了他们的虹膜和外周血样本。采用实时聚合酶链反应（PCR）方法分别分析样本中是否存在疱疹 HSV-1、HSV-2 和 CMV。采用酶联免疫吸附试验（ELISA）法测定这些病毒的 IgG 和 IgM 抗体的含量。结果CMV在淋巴管样本中的检出率为16.7%（5名患者），在外周血样本中的检出率为3.3%（1名患者）。分别有 80% 和 46.7% 的患者的 CMV 和 HSV- 1 IgG 抗体呈阳性。CMV 的 IgM 抗体阳性率为 10%。针对 HSV-1、HSV-2 和 CMV 的平均 IgM 血清抗体量分别为 2.70、1.70 和 5.47，针对这些病毒的平均 IgG 抗体量分别为 56.07、2.50 和 23.67。结论人工耳蜗植入儿童的耳周样本中存在 CMV，IgG 检测呈阳性，而外周血中不存在 CMV 基因组。这可能表明耳内曾存在这种病毒，并在听力损失中起了作用。关键词感音神经性听力损失；耳周液；巨细胞病毒；单纯疱疹病毒

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来源期刊

Auditory and Vestibular Research Medicine-Otorhinolaryngology

CiteScore

0.60

自引率

20.00%

发文量

审稿时长

12 weeks