Efficacy of Crude Extract from Streptomyces cellulosae Against Biofilm-Related Genes of Candida albicans

IF 0.5 4区医学 Q4 MICROBIOLOGY

Jundishapur Journal of Microbiology Pub Date : 2024-05-21 DOI:10.5812/jjm-145959

Chiman Hamarahim Fattah, Khattab Ahmed Mustafa Shekhany, Shwan Rachid

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引用次数: 0

Abstract

Background: Candida albicans (C. albicans) is notably pathogenic due to its ability to form biofilms that are resistant to conventional antifungal treatments. Objectives: This study aims to explore the effectiveness of Streptomyces cellulosae (S. cellulosae) extract in disrupting biofilm formation by targeting specific genes within C. albicans. Methods: The study began by isolating S. cellulosae from soil and C. albicans from clinical specimens. S. cellulosae was then cultured and fermented to produce bioactive compounds. The ability of these extracts to inhibit C. albicans biofilm formation was tested using a crystal violet assay. Additionally, the effects of the S. cellulosae extracts on the expression of biofilm-related genes in C. albicans were evaluated using quantitative real-time PCR (qRT-PCR). The growth rates of C. albicans were also measured to determine the impact of the extracts. Results: The crude extract of S. cellulosae significantly (P < 0.05) inhibited the formation of C. albicans biofilms at concentrations exceeding 0.5 µg/mL, with the inhibition becoming more pronounced at concentrations above 2.0 µg/mL. The qRT-PCR results showed significant changes in the expression of biofilm-related genes ALS1, ALS3, and EFG1 at different extract concentrations (P < 0.05). The extract also significantly affected the expression of the HWPa and BRG1 genes. Conclusions: The crude extract of Streptomyces cellulosae shows potential as a novel antibiofilm agent against C. albicans. This finding opens new avenues for research and potential therapeutic applications in combating biofilm-associated infections.

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纤维素链霉菌粗提取物对白色念珠菌生物膜相关基因的功效

背景：白色念珠菌（C. albicans）具有明显的致病性，因为它能够形成生物膜，对传统的抗真菌治疗产生抗药性。研究目的本研究旨在探讨纤维素链霉菌（S. cellulosae）提取物通过靶向白念珠菌内的特定基因破坏生物膜形成的有效性。研究方法研究首先从土壤中分离出纤维素酵母菌，并从临床样本中分离出白僵菌。然后对纤维素梭菌进行培养和发酵，以产生生物活性化合物。使用水晶紫检测法测试了这些提取物抑制白僵菌生物膜形成的能力。此外，还使用定量实时 PCR（qRT-PCR）技术评估了纤维素褐藻提取物对白僵菌生物膜相关基因表达的影响。还测量了白僵菌的生长率，以确定提取物的影响。结果纤维草粗提取物在浓度超过 0.5 µg/mL 时能显著抑制白僵菌生物膜的形成（P < 0.05），浓度超过 2.0 µg/mL 时抑制作用更加明显。qRT-PCR 结果显示，在不同提取物浓度下，生物膜相关基因 ALS1、ALS3 和 EFG1 的表达发生了显著变化（P < 0.05）。提取物还明显影响了 HWPa 和 BRG1 基因的表达。结论纤维素链霉菌的粗提取物显示出作为一种新型抗白僵菌生物膜剂的潜力。这一发现为抗击生物膜相关感染的研究和潜在治疗应用开辟了新途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Jundishapur Journal of Microbiology MICROBIOLOGY-

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Jundishapur Journal of Microbiology, (JJM) is the official scientific Monthly publication of Ahvaz Jundishapur University of Medical Sciences. JJM is dedicated to the publication of manuscripts on topics concerning all aspects of microbiology. The topics include medical, veterinary and environmental microbiology, molecular investigations and infectious diseases. Aspects of immunology and epidemiology of infectious diseases are also considered.