Comparative proteomics analysis of Shiraia bambusicola revealed a variety of regulatory systems on conidiospore formation

W. Du, Chunlong Sun, Tao Wu, Wang Li, Bin Dong, Baogui Wang, Shuai Shang, Qian Yang, Wenwen Huang, Shaopeng Chen
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Abstract

Shiraia bambusicola is a typical parasitic medicinal fungus of the family Shiraiaceae. The fruiting bodies of S. bambusicola cannot be cultivated artificially, and active substances can be effectively produced via fermentation. The mechanism of conidia production is a research hotspot in the industrial utilization and growth development of S. bambusicola. This study is the first to systematically study the proteomics of conidiospore formation from S. bambusicola. Near-spherical conidia were observed and identified by internal transcribed spacer (ITS) sequence detection. A total of 2,840 proteins were identified and 1,976 proteins were quantified in the mycelia and conidia of S. bambusicola. Compared with mycelia, 445 proteins were differentially expressed in the conidia of S. bambusicola, with 165 proteins being upregulated and 280 proteins being downregulated. The Gene Ontology (GO) annotation results of differential proteomics showed that the biological process of S. bambusicola sporulation is complex. The Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway analysis showed that the differential proteins were mainly involved in starch and sucrose metabolism, biosynthesis of secondary metabolites, microbial metabolism in diverse environments, and other processes. Our in-depth speculative analysis showed that proteins related to carbohydrate metabolism were differentially expressed in conidiospore formation of S. bambusicola, suggesting the involvement of saccharides. Conidiation may increase the synthesis and release of ethanol and polysaccharide proteins such as glycoside hydrolase (GH), suppress host immunity, and facilitate S. bambusicola to infect and colonize of the host. In-depth analysis of differential proteomes will help reveal the molecular mechanism underlying the conidiospore formation of S. bambusicola, which has strong theoretical and practical significance.
比较蛋白质组学分析揭示了分生孢子形成的多种调控系统
白头翁属(Shiraia bambusicola)是一种典型的寄生药用真菌。簕杜鹃的子实体无法人工培养,通过发酵可有效生产活性物质。分生孢子的产生机理是簕杜鹃工业利用和生长发育的研究热点。本研究首次系统研究了蚕豆菌分生孢子形成的蛋白质组学。通过内部转录间隔(ITS)序列检测,观察并鉴定了近球形的分生孢子。在 S. bambusicola 的菌丝体和分生孢子中,共鉴定出 2,840 种蛋白质,并对 1,976 种蛋白质进行了定量。与菌丝体相比,445个蛋白质在蚕蛾分生孢子中表达不同,其中165个蛋白质表达上调,280个蛋白质表达下调。差异蛋白质组学的基因本体(GO)注释结果表明,S. bambusicola 分生孢子的生物学过程是复杂的。京都基因和基因组百科全书(KEGG)代谢途径分析表明,差异蛋白主要参与淀粉和蔗糖代谢、次生代谢产物的生物合成、不同环境下的微生物代谢等过程。我们的深入推测分析表明,与碳水化合物代谢有关的蛋白质在 S. bambusicola 的分生孢子形成过程中有差异表达,这表明糖类物质参与了分生孢子的形成。分生孢子可能会增加乙醇和多糖蛋白质(如糖苷水解酶(GH))的合成和释放,抑制宿主的免疫力,促进 S. bambusicola 感染宿主并在宿主体内定殖。对差异蛋白质组的深入分析将有助于揭示蚕蛾分生孢子形成的分子机制,具有很强的理论和实践意义。
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