T Shingu, J W Karanian, H Y Kim, J A Yergey, N Salem
{"title":"Discovery of novel brain lipoxygenase products formed from docosahexaenoic acid (22:6w3).","authors":"T Shingu, J W Karanian, H Y Kim, J A Yergey, N Salem","doi":"10.1300/J251v07n03_31","DOIUrl":null,"url":null,"abstract":"<p><p>It is known that alcohol exposure leads to a decline in brain docosahexaenoate (22:6w3). We hypothesized that alcohol could stimulate the metabolism of this polyunsaturated fatty acid to bioactive products. Several oxidized products of 22:6w3 were indeed observed when rat brain homogenate was incubated with 14C22:6w3 in vitro. A similar group of metabolites was formed in vivo from 14C-22:6w3 injected into the lateral ventricle. These metabolites were characterized by thermospray- and GC/MS as well as by the synthesis of standards using purified enzymes. Platelet lipoxygenase also proved useful in identifying one of the brain metabolites and served as a source of enzyme for preparative studies. Their physiological effects on smooth muscle tone and platelet aggregation will be presented.</p>","PeriodicalId":77481,"journal":{"name":"Advances in alcohol & substance abuse","volume":"7 3-4","pages":"235-40"},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1300/J251v07n03_31","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in alcohol & substance abuse","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1300/J251v07n03_31","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 11
Abstract
It is known that alcohol exposure leads to a decline in brain docosahexaenoate (22:6w3). We hypothesized that alcohol could stimulate the metabolism of this polyunsaturated fatty acid to bioactive products. Several oxidized products of 22:6w3 were indeed observed when rat brain homogenate was incubated with 14C22:6w3 in vitro. A similar group of metabolites was formed in vivo from 14C-22:6w3 injected into the lateral ventricle. These metabolites were characterized by thermospray- and GC/MS as well as by the synthesis of standards using purified enzymes. Platelet lipoxygenase also proved useful in identifying one of the brain metabolites and served as a source of enzyme for preparative studies. Their physiological effects on smooth muscle tone and platelet aggregation will be presented.
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