Resveratrol and (-)-Epigallocatechin-3-gallate Regulate Lipid Metabolism by Activating the AMPK Pathway in Hepatocytes

Biology Pub Date : 2024-05-23 DOI:10.3390/biology13060368

Huanbin Wang, Yu An, Shahid Ali Rajput, Desheng Qi

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Abstract

The purpose of this study was to explore the effects of Res and EGCG on cell growth, cellular antioxidant levels, and cellular lipid metabolism in hepatocytes. In this experiment, leghorn male hepatoma (LMH) cells were used as hepatocytes. The results showed that 6.25–25 μM Res and EGCG had no adverse effects on cell viability and growth. Meanwhile, with the increasing dosage of Res and EGCG, the contents of total cholesterol (TC), total glyceride (TG), and malondialdehyde (MDA) in hepatocytes decreased significantly (p < 0.05), while the contents of glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), and catalase (CAT) increased significantly (p < 0.05). In addition, western blot results showed that Res and EGCG could significantly increase the expression of p-AMPK protein and reduce the expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) protein in hepatocytes (p < 0.05). Moreover, q-PCR results showed that with the increase in Res and EGCG, the expression of cholesterol- and fatty acid synthesis-related genes decreased significantly (p < 0.05). In conclusion, Res and EGCG can increase the antioxidant capacity of hepatocytes and reduce the synthesis of TC and TG in hepatocytes by activating AMPK, thereby regulating lipid metabolism in hepatocytes.

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白藜芦醇和(-)-表没食子儿茶素-3-棓酸盐通过激活肝细胞中的 AMPK 通路调节脂质代谢

本研究旨在探讨Res和EGCG对肝细胞生长、细胞抗氧化水平和细胞脂质代谢的影响。实验中，我们使用小母鸡雄性肝癌（LMH）细胞作为肝细胞。结果表明，6.25-25 μM 的 Res 和 EGCG 对细胞的活力和生长没有不良影响。同时，随着Res和EGCG用量的增加，肝细胞中总胆固醇（TC）、总甘油酯（TG）和丙二醛（MDA）的含量显著降低（p < 0.05），而谷胱甘肽过氧化物酶（GSH-Px）、总超氧化物歧化酶（T-SOD）和过氧化氢酶（CAT）的含量显著增加（p < 0.05）。此外，Western 印迹结果显示，Res 和 EGCG 能明显增加肝细胞中 p-AMPK 蛋白的表达，降低 3-羟基-3-甲基戊二酰-CoA 还原酶（HMGCR）蛋白的表达（P < 0.05）。此外，q-PCR 结果显示，随着 Res 和 EGCG 含量的增加，胆固醇和脂肪酸合成相关基因的表达量显著下降（p < 0.05）。总之，Res 和 EGCG 可提高肝细胞的抗氧化能力，并通过激活 AMPK 减少肝细胞中 TC 和 TG 的合成，从而调节肝细胞的脂质代谢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biology

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