Reference chemical database for the development and validation of in vitro alternatives to skin irritation and comparison of the performance of RhE models

IF 2.6 3区医学 Q3 TOXICOLOGY

Toxicology in Vitro Pub Date : 2024-06-01 DOI:10.1016/j.tiv.2024.105851

Nam-Hee Kang, Min Kyung Hyun, Seo Young Bang

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引用次数: 0

Abstract

After EU ban on animal testing for cosmetics in 2013, there has been an increasing global interest in alternatives test methods. To development for alternatives test method, we need to get the toxic data about in vitro and in vivo of chemicals. However, database sometimes provide limited in vivo and in vitro data on chemicals. Further, the data generated using the OECD TG439 (in vitro skin irritation) are scattered in difference databases, and it is not easy to navigate through them. Therefore, we complied ‘Reference Chemical Database System for Skin Irritation Alternative Test (RCDS-Skin Irritation)’ to allow easy, one-stop access to test chemical information. We established the systematic RCDS-Skin Irritation by collecting physiochemical properties, CAS number, human data, and in vivo (OECD TG404) data from overseas chemicals database including European Chemicals Agency (ECHA) etc., and in vitro data using Reconstructed human Epidermis (RhE) (OECD TG439). As a result, we developed the RCDS-Skin Irritation that contains information on 149 chemicals including the data we generated by performing tests using EpiDerm™ SIT, SkinEthic™ RHE and KeraSkin™ SIT.

Therefore, the RCDS-Skin Irritation established based on our study will provide insight for safety assessment of chemicals and for development of alternative test methods.

查看原文本刊更多论文

用于开发和验证体外皮肤刺激替代品以及比较 RhE 模型性能的参考化学品数据库。

2013 年欧盟禁止在化妆品中进行动物试验后，全球对替代试验方法的兴趣与日俱增。要开发替代测试方法，我们需要获得化学品的体外和体内毒性数据。然而，数据库提供的化学品体内和体外数据有时很有限。此外，使用 OECD TG439（体外皮肤刺激性）生成的数据分散在不同的数据库中，不易浏览。因此，我们编制了 "皮肤刺激替代试验参考化学品数据库系统（RCDS-Skin Irritation）"，以便于一站式获取试验化学品信息。我们通过收集欧洲化学品管理局（ECHA）等海外化学品数据库中的理化性质、CAS 编号、人体数据和体内数据（OECD TG404），以及使用重建人体表皮（RhE）的体外数据（OECD TG439），建立了系统的 RCDS-皮肤刺激性。因此，我们开发了 RCDS-皮肤刺激性，其中包含 149 种化学品的信息，包括我们使用 EpiDerm™ SIT、SkinEthic™ RHE 和 KeraSkin™ SIT 进行测试所生成的数据。因此，根据我们的研究建立的 RCDS 皮肤刺激性将为化学品的安全评估和替代测试方法的开发提供启示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.