Whole transcriptome RNA sequencing provides novel insights into the molecular dynamics of ovarian development in mud crab, Scylla paramamosain after mating

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Yang Yu , Mengqian Zhang , Dahe Wang , Zifei Xiang , Zilin Zhao , Wenxiao Cui , Shaopan Ye , Hanafiah Fazhan , Khor Waiho , Mhd Ikhwanuddin , Hongyu Ma
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Abstract

Ovarian development in animals is a complicated biological process, requiring the simultaneous coordination among various genes and pathways. To understand the dynamic changes and molecular regulatory mechanisms of ovarian development in mud crab (Scylla paramamosain), both histological observation and whole transcriptome sequencing of ovarian tissues at different mating stages were implemented in this study. The histological results revealed that ovarian development was delayed in unmated females (60 days after courtship behavior but not mating), who exhibited an oocyte diameter of 56.38 ± 15.17 μm. Conversely, mated females exhibited accelerated the ovarian maturation process, with females reaching ovarian stage III (proliferative stage) 23 days after mating and attained an average oocyte diameter of 132.19 ± 15.07 μm. Thus, mating process is essential in promoting the rapid ovarian development in mud crab. Based on the whole transcriptome sequencing analysis, a total of 518 mRNAs, 1502 lncRNAs, 18 circRNAs and 151 miRNAs were identified to be differentially expressed between ovarian tissues at different mating stages. Notably, six differentially expressed genes (DEGs) associated with ovarian development were identified, including ovary development-related protein, red pigment concentrating hormone receptor, G2/mitotic-specific cyclin-B3-like, lutropin-chorio gonadotropic hormone receptor, renin receptor, and SoxB2. More importantly, both DEGs and targets of differentially expressed non-coding RNAs (DEncRNAs) were enriched in renin-angiotensin system, TGF-β signaling, cell adhesion molecules, MAPK signaling pathway, and ECM-receptor interaction, suggesting that these pathways may play significant roles in the ovarian development of mud crabs. Moreover, competition endogenous RNA (ceRNA) networks were constructed while mRNAs were differentially expressed between mating stages were involved in Gene Ontology (GO) biological processes such as developmental process, reproduction, and growth. These findings could provide solid foundations for the future development of female mud crab maturation enhancement strategy, and improve the understanding of the ovarian maturation process in crustaceans.

Abstract Image

全转录组 RNA 测序为了解泥蟹交配后卵巢发育的分子动态提供了新的视角
动物卵巢的发育是一个复杂的生物学过程,需要各种基因和通路的同时协调。为了解泥蟹卵巢发育的动态变化和分子调控机制,本研究对不同交配阶段的卵巢组织进行了组织学观察和全转录组测序。组织学结果显示,未交配雌蟹(求偶行为后 60 天,但未交配)的卵巢发育延迟,卵母细胞直径为 56.38 ± 15.17 μm。相反,交配后的雌性卵巢成熟过程加快,交配后 23 天达到卵巢 III 期(增殖期),卵母细胞平均直径为 132.19 ± 15.07 μm。因此,交配过程对促进泥蟹卵巢的快速发育至关重要。基于全转录组测序分析,共鉴定出518个mRNA、1502个lncRNA、18个circRNA和151个miRNA在不同交配阶段的卵巢组织间存在差异表达。值得注意的是,研究发现了六个与卵巢发育相关的差异表达基因(DEGs),包括卵巢发育相关蛋白、红色素浓缩激素受体、G2/有丝分裂期特异性细胞周期蛋白-B3-like、促性腺激素-绒毛膜促性腺激素受体、肾素受体和SoxB2。更重要的是,差异表达的非编码RNA(DEncRNAs)的DEGs和靶标都富集在肾素-血管紧张素系统、TGF-β信号传导、细胞粘附分子、MAPK信号传导通路和ECM-受体相互作用中,表明这些通路可能在大闸蟹卵巢发育过程中发挥重要作用。此外,还构建了竞争性内源性 RNA(ceRNA)网络,而不同交配阶段差异表达的 mRNA 参与了基因本体(GO)生物学过程,如发育过程、繁殖和生长。这些发现可为未来开发雌性泥蟹成熟增强策略提供坚实的基础,并增进对甲壳类卵巢成熟过程的了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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