Telocytes: Detection, Visualization, Tissue Dissociation, and Tamoxifen-Induction of Transgenic Mice.

Q4 Biochemistry, Genetics and Molecular Biology
Marco Canella, Michal Shoshkes-Carmel
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引用次数: 0

Abstract

Telocytes, distinctive interstitial cells, have recently emerged as crucial components of the stem-cell niche in the intestine. Notably, telocytes are distinguished by their extremely long cellular protrusions extending hundreds of microns from the cell body, forming an interconnected network along the intestinal crypt villus axis. Due to these unique cellular characteristics, there is a need for tailored working protocols to effectively characterize and target telocytes. Here, we outline advanced and progressive protocols for tissue fixation, dissociation, visualization, and the use of tamoxifen-induced transgenic mouse models to specifically target telocytes.

端细胞:转基因小鼠的检测、可视化、组织分离和他莫昔芬诱导。
端粒细胞是一种独特的间质细胞,最近已成为肠道干细胞生态位的重要组成部分。值得注意的是,端粒细胞的特点是细胞突起极长,从细胞体延伸数百微米,沿着肠隐窝绒毛轴形成一个相互连接的网络。由于这些独特的细胞特征,需要量身定制的工作方案来有效表征和靶向端粒细胞。在这里,我们概述了先进的渐进式组织固定、解离、可视化方案,以及使用他莫昔芬诱导的转基因小鼠模型来特异性靶向端粒细胞。
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来源期刊
Methods in molecular biology
Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
2.00
自引率
0.00%
发文量
3536
期刊介绍: For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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