Effect of culture hydrodynamics on Arthrospira platensis production using a single-use photobioreactor system through a CFD supported approach.

IF 2.5 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
S Furkan Demirden, Barıs Erdogan, Deniz Şenyay Öncel, Suphi S Oncel
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引用次数: 0

Abstract

Laboratory scale conventional single-use bioreactor was used to investigate the effect of different stirrer speeds on the Arthrospira platensis (Spirulina platensis) culture. Experiments were handled in two steps. First step was the selection of the stirring speeds, which was simulated via using CFD, and the second was the long term cultivation with the selected speed. During 10 days of batches as the first step, under identical culture conditions, stirrer speed of 230 rpm gave higher results, compared to 130 and 70 rpm, with respect to dry biomass weight, absorbance value (AB) and chlorophyll-a concentration. Volumetric productivity during the growth phase of the cultures were calculated as 0.39 ± 0.03, 0.28 ± 0.01, and 0.19 ± 0.02 g L-1 d-1, from the fast to the slower speeds. According to the results a 17 day batch was handled with 230 rpm in order to monitor the effects on the culture. The culture reached a volumetric productivity of 0.33 ± 0.04 g L-1 d-1. Statistical analysis showed the significance of the parameters related with the stirring speed.

通过 CFD 支持方法研究培养液流体力学对使用一次性光生物反应器系统生产节肢动物的影响。
使用实验室规模的传统一次性生物反应器研究不同搅拌速度对板蓝根节旋藻(螺旋藻)培养的影响。实验分两步进行。第一步是选择搅拌速度,通过 CFD 进行模拟;第二步是用选定的速度进行长期培养。第一步是在相同的培养条件下,分批进行 10 天的培养,在干生物量重量、吸光度值(AB)和叶绿素-a 浓度方面,230 转/分钟的搅拌速度比 130 转/分钟和 70 转/分钟的搅拌速度得到了更高的结果。根据计算,在培养物的生长阶段,从快转速到慢转速的体积生产率分别为 0.39 ± 0.03、0.28 ± 0.01 和 0.19 ± 0.02 g L-1 d-1。根据计算结果,用 230 转/分钟的转速处理了 17 天的批次,以监测对培养物的影响。培养物的体积生产率为 0.33 ± 0.04 g L-1 d-1。统计分析表明,与搅拌速度相关的参数具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biotechnology Progress
Biotechnology Progress 工程技术-生物工程与应用微生物
CiteScore
6.50
自引率
3.40%
发文量
83
审稿时长
4 months
期刊介绍: Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.
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