Assessment of anti-diabetic properties of Ziziphus oenopolia (L.) wild edible fruit extract: In vitro and in silico investigations through molecular docking analysis

IF 2.1 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Open Chemistry Pub Date : 2024-05-14 DOI:10.1515/chem-2024-0032

R. Shunmuga Vadivu, Senthil Bakthavatchalam, Vasthi Gnana Rani, Abdurahman Hajinur Hirad, Zhi-Hong Wen, Chien-Han Yuan, Ramachandran Vinayagam

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引用次数: 0

Abstract

Globally, healthcare is concerned about the rising prevalence of type 2 diabetes. Phytochemicals from medicinal plants have shown great promise in improving human health. The present study aimed to determine the secondary metabolites of Ziziphus oenopolia (L.) fruit extract that contribute to its anti-diabetic activity. The anti-diabetic properties were assessed by in vitro and in silico approaches using α-amylase and α-glucosidase inhibitory assays. Gas chromatography and mass spectroscopy analyses were used to profile Z. oenopolia fruit contents, and a total of four bioactive chemicals and eight phytocompounds were tentatively identified, including flavonoids, terpenoids, phenols, steroids, tannins, and saponins. The Z. oenopolia fruit hydroalcoholic extract inhibits α-amylase and α-glucosidase enzymes in a dose-dependent manner (IC50 = 328.76 and 337.28 µg/mL, R 2 = 0.979 and 0.981). Additionally, phytochemicals found in Z. oenopolia fruit exhibit the ability to inhibit anti-diabetic targets, specifically α-amylase and α-glucosidase (2QV4 vs 3A4A; correlation coefficient, r = 0.955), as demonstrated by computational analysis. This establishes the fruit as a promising and environmentally friendly option for treating hyperglycemia, highlighting the positive correlation between anti-diabetic objectives.

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野生食用果实提取物的抗糖尿病特性评估：通过分子对接分析进行体外和硅学研究

在全球范围内，2 型糖尿病发病率的上升引起了医疗保健界的关注。从药用植物中提取的植物化学物质在改善人类健康方面大有可为。本研究旨在确定 Ziziphus oenopolia（L.）果实提取物中有助于其抗糖尿病活性的次生代谢物。通过体外和硅学方法，使用α-淀粉酶和α-葡萄糖苷酶抑制试验评估了其抗糖尿病特性。利用气相色谱和质谱分析法对 Z. oenopolia 果实的成分进行了分析，共初步鉴定出四种生物活性化学物质和八种植物化合物，包括黄酮类、萜类、酚类、甾体、单宁和皂苷。油橄榄果实水醇提取物对α-淀粉酶和α-葡萄糖苷酶的抑制作用呈剂量依赖性（IC50 = 328.76 和 337.28 µg/mL，R 2 = 0.979 和 0.981）。此外，计算分析表明，在泽泻果实中发现的植物化学物质具有抑制抗糖尿病目标的能力，特别是α-淀粉酶和α-葡萄糖苷酶（2QV4 与 3A4A；相关系数，r = 0.955）。这证明该水果是治疗高血糖的一种有前途的环保选择，突出了抗糖尿病目标之间的正相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

3.80

自引率

4.30%

发文量

审稿时长

6 weeks

期刊介绍： Open Chemistry is a peer-reviewed, open access journal that publishes original research, reviews and short communications in the fields of chemistry in an ongoing way. The central goal is to provide a hub for researchers working across all subjects to present their discoveries, and to be a forum for the discussion of the important issues in the field. The journal is the premier source for cutting edge research in fundamental chemistry and it provides high quality peer review services for its authors across the world. Moreover, it allows for libraries everywhere to avoid subscribing to multiple local publications, and to receive instead all the necessary chemistry research from a single source available to the entire scientific community.