L. Khmelevtsova, M. Klimova, Shorena Karchava, T. Azhogina, E. Polienko, A. Litsevich, Elena Chernyshenko, Margarita Khammami, I. Sazykin, M. Sazykina
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引用次数: 0
Abstract
Pesticides and fertilizers used in agriculture can negatively affect the soil, increasing its toxicity. In this work, a battery of whole-cell bacterial lux-biosensors based on the E. coli MG1655 strain with various inducible promoters, as well as the natural luminous Vibrio aquamarinus VKPM B-11245 strain, were used to assess the effects of agrochemical soil treatments. The advantages of using biosensors are sensitivity, specificity, low cost of analysis, and the ability to assess the total effect of toxicants on a living cell and the type of their toxic effect. Using the V. aquamarinus VKPM B-11245 strain, the synergistic effect of combined soil treatment with pesticides and mineral fertilizers was shown, which led to an increase in the overall (integral) toxicity of soils higher than that of the individual application of substances. Several probable implementation mechanisms of agrochemical toxic effects have been discovered. DNA damage caused by both SOS response induction and alkylation, oxidative stress due to increased superoxide levels, and damage to cellular proteins and membranes are among them. Thus, the usage of biosensors makes it possible to assess the cumulative effect of various toxicants on living organisms without using expensive chemical analyses.
农业中使用的农药和化肥会对土壤产生负面影响,增加其毒性。在这项工作中,使用了一系列基于大肠杆菌 MG1655 菌株和天然发光弧菌 VKPM B-11245 菌株的全细胞细菌勒克斯生物传感器来评估农用化学品对土壤的影响。使用生物传感器的优点是灵敏度高、特异性强、分析成本低,而且能够评估毒物对活细胞的总体影响及其毒害作用的类型。使用 V. aquamarinus VKPM B-11245 菌株研究表明,农药和矿物肥料联合处理土壤会产生协同效应,导致土壤总体(整体)毒性增加,高于单独施用物质的毒性。现已发现农用化学品毒性效应的几种可能的实施机制。其中包括 SOS 反应诱导和烷基化引起的 DNA 损伤、超氧化物含量增加导致的氧化应激以及细胞蛋白质和细胞膜损伤。因此,使用生物传感器可以评估各种毒物对生物体的累积效应,而无需使用昂贵的化学分析方法。
期刊介绍:
Chemosensors (ISSN 2227-9040; CODEN: CHEMO9) is an international, scientific, open access journal on the science and technology of chemical sensors published quarterly online by MDPI.