TO STUDY THE EFFECT OF DIFFERENT DOSES OF RADIATION EXPOSURE ON THE LEVEL OF MINERALIZATION IN DIFFERENT AREAS OF HARD DENTAL TISSUES BY RAMAN FLUORESCENCE

Abstract

Subject. Changes in the mineralization of hard tissues of teeth under the influence of radiation factor. Objectives. To study the effect of radiation factor on the level of mineralization of hard dental tissues by Raman fluorescence. Methodology. The study was conducted at the Department of Orthopedic Dentistry and Orthodontics of the SUSMU. The study was conducted on clinically removed teeth. All teeth were divided into three groups depending on the amount of radiation exposure (2 Gy, 70 Gy, 110 Gy). The main research method was the study of raman fluorescence of tooth sections on the hardware and software complex "InSpectr" with diagnostic sensitivity for the integral concentration of aerobic-anaerobic microbial suspension up to 109 CFU/ml. Teeth were examined in three areas (neck, equator, cutting edge) before and after radiation exposure. Results. Raman fluorescence diagnostics of the tooth surface made it possible to visually see the difference in mineralization in digitized indicators. For example, according to the indicators before and after exposure to the radiation factor, it is clear that the mineralization indicators, regardless of the dose, had no significant differences. At the same time, there are significant differences in the level of mineralization in the area of different tooth settings (equator, cervical region, cutting edge). Conclusion. In different areas of the tooth surface, the level of mineralization of hard tissues differs. The smallest is observed in the area of the neck of the teeth (incisors, y = 145 ± 1.5, x = 963 cm-1, canines, y = 141 ± 1.1, x = 963 cm-1, premolars, y = 142 ± 1.8 , x = 963 cm-1, molars, y = 143 ± 1.3, x = 963 cm-1), middle, in the area of the cutting edge and occlusal surface (incisors, y = 374 ± 1.7, x = 963 cm-1, canines, y = 377 ± 1.3, x = 963 cm-1, premolars, y = 375 ± 1.2, x = 963 cm-1, molars, y = 375 ± 1.1, x = 963 cm-1), and maximum, in the equator region (incisors, y = 413 ± 1.1, x = 963 cm-1, canines, y = 414 ± 1.9, x = 963 cm-1, premolars, y = 415 ± 1.7, x = 963 cm-1, molars, y = 419 ± 1.6, x = 963 cm-1). The method of Raman-fluorescence diagnostics makes it possible to detect changes in mineralization due to the potential difference in the areas of hard tooth tissues in various areas (neck, equator, cutting edge) of all functional groups of teeth (incisors, canines, premolars, molars) both before and after direct radiation exposure, direct radiation exposure does not significantly change the level of mineralization of hard dental tissues, regardless of the dose applied in all functional groups (incisors, canines, premolars, molars), in all areas of the teeth (equator, cutting edge, cervical region).