Microbiological identification of some culturable native strains associated with the digestive tract in Panaque cochliodon

Juan David Cano-Gil, Luz Adriana Gutiérrez-Ramírez, Carlos A. David-Ruales, S. Pardo-Carrasco, Valentina Jaramillo-Ruiz, Manuela Arboleda-Restrepo
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Abstract

Objective. To characterize by microbiology the native strains associated to the intestinal tract of the species Panaque cochliodon. Materials and methods. Three adult specimens captured in the Magdalena River that were transported and sacrificed under animal welfare regulations were used. Dissection of the intestinal tract was performed, obtaining samples for microbiological isolation, using culture media, purifying the microorganisms, performing metabolic biochemical tests and API 20E (Biomeriux) tests for their identification. Results. Information on the microbial population structure was obtained, reporting the phylum Proteobacteria with the species: Pantoea sp, Erwinia sp, Providencia stuarti, Providencia alcalifaciens, Serratia ficaria, Citrobacter koseri and the phylum Firmicutes with the species: Bacillus sphaericus, Bacillus subtilis, Bacillus thurigiensis, Bacillus mycoides, Bacillus coagulans and Bacillus circulans. Conclusion. The predominant culturable microorganisms in P. cohliodon belong to the phylum Proteobacteria and the phylum Firmicutes.
与蟠龙消化道相关的一些可培养本地菌株的微生物鉴定
目的通过微生物学方法鉴定与蟠龙鱼肠道相关的本地菌株。材料和方法。使用了在马格达莱纳河捕获的三条成年标本,根据动物福利法规,这些标本被运送并牺牲。对标本的肠道进行解剖,获取样本进行微生物分离,使用培养基,纯化微生物,进行代谢生化测试和 API 20E(Biomeriux)测试以鉴定微生物。结果获得了微生物种群结构的信息,报告了变形菌门的物种:变形菌门有 Pantoea sp、Erwinia sp、Providencia stuarti、Providencia alcalifaciens、Serratia ficaria、Citrobacter koseri,固有菌门有芽孢杆菌(Bacillus sphaericus)、枯草芽孢杆菌(Bacillus subtilis)、苏云金芽孢杆菌(Bacillus thurigiensis)、霉形芽孢杆菌(Bacillus mycoides)、凝结芽孢杆菌(Bacillus coagulans)和循环芽孢杆菌(Bacillus circulans)。结论共生藻中的主要可培养微生物属于变形菌门和固着菌门。
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