DESIGN, DEVELOPMENT AND IMPROVEMENT OF AN EMULGEL CONTAINING SILVER NANOPARTICLES AND VITAMIN D-3 FOR ITS POTENTIAL TO ACCELERATE THE HEALING OF WOUND

Q2 Pharmacology, Toxicology and Pharmaceutics

International Journal of Applied Pharmaceutics Pub Date : 2024-05-07 DOI:10.22159/ijap.2024v16i3.50344

Rishu Yadav, Narendra Kumar Pandey, Rajiv Kukkar

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引用次数: 0

Abstract

Objective: The aim of this research work was to prepare a topical emulgel based dosage form incorporated with vitamin D-3 and silver nanoparticles to reduce the wound healing time in any kind of wound. Methods: Central Composite Design (CCD) was applied for the optimization of emulgel by using Design expert software. Three responses (pH, viscosity, and in vitro drug release) and two factors (Carbopol concentration and stirring duration) were chosen, and Statistical Analysis of Variance (ANOVA) revealed that all the factors were significantly affecting the responses. Silver Nanoparticles (SNPs) was prepared with Green Tea Extract (GTE) and evaluated for particle size, Poly Dispersity Index (PDI), zeta potential and Fourier Transform Infra-red (FTIR) spectroscopy and revealed that SNPs of desired range and stability have been synthesized. Here excision wound model was used to evaluate the wound healing activity of formulation in vivo. Results: Maximum in vitro release 88.2±2.1 has shown by the optimized formulation F13, pH and viscosity were also found in optimum range i.e., 6.2±0.4 and 1672±33 respectively, followed by Korsmeyer and Peppas model. Total eight groups were designed for animal study and silver sulphadiazine was used as marketed formulation. F13 formulation was further evaluated for in vivo data, it was revealed that emulgel loaded with high dose of vitamin D-3 along with silver nanoparticles has shown 100.5±1.7% wound contraction, while marketed formulation has shown 103.7±1.1% wound contraction, which was much similar with test formulation. Cytotoxic cell study was done using assay on chicken egg, formulation has not shown any cytotoxic behaviour like haemolysis and cell damage on chick embryo’s blood vessels. Accelerated stability study of the optimized formulation was also performed to check whether the formulation was stable or not and it was revealed that optimized formulation was found stable for the period of six months. Conclusion: It was revealed that emulgel loaded with high dose of vitamin D-3 and SNPs found suitable to accelerate wound healing and showed almost similar response in wound contraction on comparison with marketed formulation. This emulgel promised to controlled the delivery of the drug for the longer duration.

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设计、开发和改进含银纳米粒子和维生素 d-3 的凝胶，以提高其加速伤口愈合的潜力

研究目的本研究工作旨在制备一种含有维生素 D-3 和纳米银颗粒的外用乳胶剂型，以缩短各种伤口的愈合时间：方法：利用 Design expert 软件，采用中央复合设计（CCD）对润肤凝胶进行优化。选择了三个响应（pH 值、粘度和体外药物释放）和两个因素（Carbopol 浓度和搅拌持续时间），统计方差分析（ANOVA）显示，所有因素都对响应有显著影响。用绿茶提取物（GTE）制备了银纳米颗粒（SNPs），并对其粒度、聚分散指数（PDI）、ZETA电位和傅立叶变换红外光谱（FTIR）进行了评估，结果表明合成的 SNPs 具有所需的范围和稳定性。在此，使用切除伤口模型来评估制剂在体内的伤口愈合活性：结果：优化配方 F13 的体外最大释放量为 88.2±2.1，pH 值和粘度也在最佳范围内，分别为 6.2±0.4 和 1672±33，其次是 Korsmeyer 和 Peppas 模型。共设计了八组动物实验，磺胺嘧啶银被用作市场上的制剂。对 F13 制剂进行了进一步的体内数据评估，结果显示，含有高剂量维生素 D-3 和银纳米粒子的凝胶显示出 100.5±1.7% 的伤口收缩率，而市售制剂显示出 103.7±1.1% 的伤口收缩率，与试验制剂非常相似。细胞毒性研究是在鸡卵上进行的，配方没有显示出任何细胞毒性行为，如溶血和对鸡胚血管的细胞损伤。此外，还对优化配方进行了加速稳定性研究，以检查配方是否稳定，结果表明优化配方在 6 个月内保持稳定：结论：研究发现，含有高剂量维生素 D-3 和 SNP 的凝胶适合加速伤口愈合，与市场上销售的配方相比，它在伤口收缩方面表现出几乎相似的反应。这种凝胶有望在更长的时间内控制药物的输送。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Applied Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

CiteScore

1.40

自引率

0.00%

发文量

219

期刊介绍： International Journal of Applied Pharmaceutics (Int J App Pharm) is a peer-reviewed, bimonthly (onward March 2017) open access journal devoted to the excellence and research in the pure pharmaceutics. This Journal publishes original research work that contributes significantly to further the scientific knowledge in conventional dosage forms, formulation development and characterization, controlled and novel drug delivery, biopharmaceutics, pharmacokinetics, molecular drug design, polymer-based drug delivery, nanotechnology, nanocarrier based drug delivery, novel routes and modes of delivery; responsive delivery systems, prodrug design, development and characterization of the targeted drug delivery systems, ligand carrier interactions etc. However, the other areas which are related to the pharmaceutics are also entertained includes physical pharmacy and API (active pharmaceutical ingredients) analysis. The Journal publishes original research work either as a Original Article or as a Short Communication. Review Articles on a current topic in the said fields are also considered for publication in the Journal.