BIOSYNTHESIS, CHARACTERIZATION AND EVALUATION OF SILVER NANOPARTICLES FROM THE LEAF EXTRACT OF PREMNA INTIGRIFOLIA L. AS A POTENTIAL ANTICANCER AGENT

Q2 Pharmacology, Toxicology and Pharmaceutics

International Journal of Applied Pharmaceutics Pub Date : 2024-05-07 DOI:10.22159/ijap.2024v16i3.50260

SPANDANA K., Arun K. Shettar, R. S.

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引用次数: 0

Abstract

Objective: In this study, plant-based silver nanoparticles were synthesized and characterized from Premna integrifolia leaf extract to test the viability towards anticancer properties. Methods: Preliminary identification of silver nanoparticles was validated by Visual observation and confirmed for the characterization by Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX) and Fourier-transform Infrared Spectroscopy (FTIR) analysis. Further synthesized nanoparticles were evaluated against non-small lung cancer cells (A549) by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. Results: Aqueous leaf extract of Premna intigrifolia was synthesized for silver nanoparticles and showed an average size from 35nm to 100 nm through SEM studies. EDX showed a strong signal confirming the formation of silver nanoparticles in the metallic silver region at 5Kev, and the FTIR spectrum showed changes in some peaks of the aqueous extract with functional groups. The newly synthesized silver nanoparticles showed significant anticancer properties targeting lung cancer A549 cell line against standard drug Epotoside with a 50% Inhibitory Concentration (IC50) value of 78.431 µg. Conclusion: The results affirm that biosynthesized silver nanoparticles can be used as an alternative to chemical medicines to cure cancer.

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作为一种潜在抗癌剂的无花果树叶提取物银纳米粒子的生物合成、表征和评估

研究目的本研究从 Premna integrifolia 叶提取物中合成了植物银纳米粒子，并对其进行了表征，以测试其抗癌活性。方法：通过观察和验证银纳米颗粒的初步鉴定：通过肉眼观察对银纳米粒子进行初步鉴定，并通过扫描电子显微镜（SEM）、能量色散 X 射线光谱（EDX）和傅立叶变换红外光谱（FTIR）分析确认其特征。通过 MTT（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑）检测法评估了进一步合成的纳米颗粒对非小肺癌细胞（A549）的作用：通过扫描电镜研究，银纳米粒子的平均尺寸为 35 纳米至 100 纳米。EDX 在 5Kev 处显示出强烈的信号，证实在金属银区域形成了银纳米粒子，傅立叶变换红外光谱显示出水提取物中一些带有官能团的峰值发生了变化。新合成的银纳米粒子对肺癌 A549 细胞株具有显著的抗癌作用，其 50%的抑制浓度（IC50）为 78.431 µg：研究结果表明，生物合成的银纳米粒子可替代化学药物治疗癌症。

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来源期刊

International Journal of Applied Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (miscellaneous)

CiteScore

1.40

自引率

0.00%

发文量

219

期刊介绍： International Journal of Applied Pharmaceutics (Int J App Pharm) is a peer-reviewed, bimonthly (onward March 2017) open access journal devoted to the excellence and research in the pure pharmaceutics. This Journal publishes original research work that contributes significantly to further the scientific knowledge in conventional dosage forms, formulation development and characterization, controlled and novel drug delivery, biopharmaceutics, pharmacokinetics, molecular drug design, polymer-based drug delivery, nanotechnology, nanocarrier based drug delivery, novel routes and modes of delivery; responsive delivery systems, prodrug design, development and characterization of the targeted drug delivery systems, ligand carrier interactions etc. However, the other areas which are related to the pharmaceutics are also entertained includes physical pharmacy and API (active pharmaceutical ingredients) analysis. The Journal publishes original research work either as a Original Article or as a Short Communication. Review Articles on a current topic in the said fields are also considered for publication in the Journal.