Natural Deep Eutectic Solvent-Based Ultrasound-Assisted Extraction of Flavonoids from Fagopyrum tataricum Bran

Separations Pub Date : 2024-05-08 DOI:10.3390/separations11050145

Zhou Xu, Xiaomei Da, Jipeng Qu, Shiming Xiao

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Abstract

In this study, eleven kinds of flavonoids were identified from F. tataricum bran (FTB) by UPLC-Q-TOF-MS, and HPLC-DAD analysis revealed that four compounds, including rutin, quercetin, kaempferol, and nicotiflorin, were the most significant components. Subsequently, natural deep eutectic solvent-based ultrasound-assisted extraction (NADES-UAE) was employed to extract flavonoids from FTB. Among the six kinds of NADES prepared, choline chloride–ethylene glycol (ChCl-EG) was identified as a promising candidate for extracting flavonoids due to its superior extraction performance. The extraction conditions were statistically investigated using response surface methodology conducted by Box-Behnken design (BBD). The optimal operational conditions were as follows: ultrasonic time 268 s, ultrasonic temperature 76 °C, and liquid–solid ratio 43 mL/g, which resulted in a high total flavonoid yield of 40.29 mg/g. Afterwards, the efficient extraction mechanism of NADES-UAE was comprehensively explored through FT-IR spectra, COSMO model, and microstructural analysis. In conclusion, NADES-UAE extraction is considered a green, efficient, and sustainable method for FTB flavonoids.

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基于天然深共晶溶剂的超声波辅助提取鞑靼糙米中的黄酮类化合物

本研究采用UPLC-Q-TOF-MS鉴定了鞑靼藜麦麸（FTB）中的11种黄酮类化合物，HPLC-DAD分析表明，芦丁、槲皮素、山柰酚和烟花色苷等4种化合物是最主要的成分。随后，采用基于天然深共晶溶剂的超声辅助萃取（NADES-UAE）技术从枸杞多糖中提取黄酮类化合物。在所制备的六种 NADES 中，氯化胆碱-乙二醇（ChCl-EG）因其优异的萃取性能被认为是萃取黄酮类化合物的理想候选溶剂。采用方框-贝肯设计（BBD）的响应面方法对萃取条件进行了统计研究。最佳操作条件为：超声时间 268 s，超声温度 76 °C，液固比 43 mL/g，总黄酮产量高达 40.29 mg/g。随后，通过傅立叶变换红外光谱、COSMO模型和微观结构分析，全面探讨了NADES-UAE的高效提取机理。总之，NADES-UAE 是一种绿色、高效、可持续的萃取枸橼酸黄酮类化合物的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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