Huanglian Jiedu Decoction Inhibits LPS-induced Neuroinflammation and Attenuates the  Activation of NLRP3 Inflammasome in  BV2 Cells

Pharmacognosy Magazine Pub Date : 2024-05-09 DOI:10.1177/09731296241244575

Junli Wang, Rui Wang, Yaming Du, Xinzi Xu, Xin Liu, Wei Shao, Guohua Chen

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Abstract

Background: Huanglian Jiedu decoction (HLJDD) is a classical herbal formula for treating inflammatory diseases with Traditional Chinese Medicine (TCM). However, the impacts of HLJDD on neuroinflammation and the precise mechanisms involved remain poorly understood. Purpose: In the present study, HLJDD was found to inhibit lipopolysaccharide (LPS) induced neuroinflammatory responses in BV2 microglia via regulating NLRP3 inflammasome activation. Methods: Anti-inflammatory activity was evaluated with ELISA assay and the polarization changes were observed using immunofluorescent staining. The levels of NLRP3 inflammasome activation were determined using qPCR and Western blotting. Results: The administration of HLJDD successfully countered LPS-induced neuroinflammatory responses by attenuating microglial activation and fostering a phenotypic conversion from the pro-inflammatory M1 to the anti-inflammatory M2. . Additionally, HLJDD treatment demonstrated inhibition of NLRP3 inflammasome activation. Conclusion: The findings of the present study may provide insights into how HLJDD could be a therapeutic drug for neuroinflammatory diseases associated with regulating NLRP3 inflammasome and microglial activation.

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黄连解毒片可抑制 LPS 诱导的神经炎症并减轻 BV2 细胞中 NLRP3 炎症小体的激活

背景：黄连解毒汤（HLJDD）是中医治疗炎症性疾病的经典中药配方。然而，人们对 HLJDD 对神经炎症的影响及其确切机制仍知之甚少。目的：本研究发现，HLJDD可通过调节NLRP3炎性体的激活来抑制脂多糖（LPS）诱导的BV2小胶质细胞神经炎症反应。方法：用酶联免疫吸附试验评估抗炎活性，用免疫荧光染色观察极化变化。用 qPCR 和 Western 印迹法测定 NLRP3 炎性体的活化水平。结果：服用 HLJDD 可减轻小胶质细胞的活化，促进表型从促炎性 M1 向抗炎性 M2 转换，从而成功对抗 LPS 诱导的神经炎症反应。此外，HLJDD 还能抑制 NLRP3 炎性体的激活。结论本研究的结果可能有助于深入了解 HLJDD 如何成为一种治疗药物，用于治疗与 NLRP3 炎性体和小胶质细胞活化调节相关的神经炎性疾病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Pharmacognosy Magazine

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Huanglian Jiedu Decoction Inhibits LPS-induced Neuroinflammation and Attenuates the Activation of NLRP3 Inflammasome in BV2 Cells

Abstract

Huanglian Jiedu Decoction Inhibits LPS-induced Neuroinflammation and Attenuates the  Activation of NLRP3 Inflammasome in  BV2 Cells