Metaanalysis of Public Wastewater Metagenomes: Revealing the Influence of Climatic Variations on the Abundance of the Bacterial Members

Abstract

As per the present trend, earth may experience severe hike in temperature by 2040. Therefore, global surveillance is required to combat against the upcoming climatic extremities. Emerging and re-emerging infectious diseases are part of the key pressing issues which can affect millions of people worldwide. Wastewater associated metagenomic data representing the wastewater samples collected from sewer networks in Newcastle (United Kingdom); Zimbabwe (Africa) and West Bengal (India), available in public databases were collected. It was observed that of the top 10 abundant genera, the prevalent genera in samples from United Kingdom, Africa, India were Desulfitobacterium, Prevotella, Sulfurospirillum respectively. By comparing all the three samples under study; it was observed that three microbial genera Bacteroides, Dechloromonas and Faecalibacterium were common throughout the samples. Metagenome-wide association studies were conducted to link microbes in the sample to the host disorders. Our study could predict overexpression of several enriched pathways like Vancomycin resistance, Platinum drug resistance across the samples. These findings prove efficacies of the community genomics approach to serve as a public health monitoring tool through detection of spatial variation as well as degree of commonness in wastewater bacterial profiles and also through the prediction of the pathogenic quantum of wastewater systems.